ING4基因表达对乳腺癌细胞生长抑制作用的研究  

The inhibitory effects of exogenous ING4 gene expression on the growth of breast cancer cells

作　　者：曹芳[1] 魏钦俊[1] 鲁雅洁[1] 潘梅[1] 曹新[1] 

机构地区：[1]南京医科大学生物技术系,南京210029

出　　处：《肿瘤》2008年第4期301-304,共4页Tumor

摘　　要：目的:研究人ING4基因对乳腺癌细胞MCF-7的生长抑制作用。方法:通过实时荧光定量-PCR(real-time fluorogen-tic quantitative-PCR,RFQ-PCR)检测3种乳腺癌细胞株中ING4 mRNA的表达水平;ING4基因的表达质粒转染MCF-7细胞;MTT法和FCM法检测ING4基因对MCF-7细胞增殖的影响;Annexin-Ⅴ/PI双染法观察细胞凋亡情况;RT-PCR法分析转染ING4后p53、p21及bax基因的转录表达情况。结果:在3种乳腺癌细胞MCF-7、MDA-MB-435和MDA-MB-231中ING4的表达均明显低于正常乳腺组织。转染ING4表达载体的MCF-7细胞较对照组细胞生长速度减慢(P<0.05);细胞周期中G1期比例增加,S期比例减少;细胞凋亡率升高。p53的表达未见明显变化,而p21和bax表达显著上调。结论:ING4与乳腺癌的发生发展具有一定相关性;高表达ING4基因能够抑制人乳腺癌细胞MCF-7的生长。Objective：To observe the inhibitory effects of ING4 gene expression on the growth of MCF-7 cells. Methods： ING4 mRNA expression in three breast cancer lines was detected by real-time fluorogentic quantitative-PCR （RFQ-PCR）. pcDNA3. 1 （ ＋ ） ING4 was transfected into MCF-7 cells. The proliferation activity of MCF-7 cells was measured by MTT assay and flow cytometry. Cell apoptotic ratios were analyzed by Annexin-V/PI double staining. Results：The expression of ING4 was markedly lower in MCF-7, MDA-MB-435, and MDA-MB-231 cells compared with normal breast tissues. Over expression of ING4 slowed down proliferation speed of MCF-7 cells, increased the cell proportion in G1 phase and decreased the proportion in S phase （ P 〈 0. 05 ）. The apoptotic ratio of MCF-7 cells was significantly increased compared to control group （P 〈 0.05 ）. RT-PCR revealed that expressions of p21 and bax gene were all up-regulated but p53 had no change. Conclusion：ING4 was associated with tumorigenesis of breast cancer, Over expression of ING4 inhibited the proliferation of human breast cancer MCF-7 cells.

关 键 词：乳腺肿瘤 转染 基因 肿瘤抑制 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]