PI3K／AKT／p-AKT在宫颈癌组织中的表达及其与Ki67关系的研究  

Expressions of PI3K/AKT/p-AKT in cervical intraepithelial neoplasia and cervical carcinoma and their relationship with Ki67

作　　者：廖书杰[1] 袁兵[2] 胡晓继[1] 周蓉 卢运萍[1] 王世宣[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430032 [2]云南省第一人民医院呼吸内科,昆明650000 [3]华中科技大学同济医学院附属同济医院肾内科,武汉430030

出　　处：《肿瘤》2008年第4期317-321,共5页Tumor

基　　金：国家重点基础研究发展计划项目(九七三计划)(编号:2002CB513100)

摘　　要：目的:通过检测PI3K、AKT、P-AKT和Ki67在宫颈癌中的表达,探讨其与宫颈病变发生发展的关系及相关的临床意义。方法:应用免疫组织化学SP法检测宫颈癌组织及其正常组织中PI3K、AKT、p-AKT和Ki67的表达。结果:(1)宫颈癌中PI3K、AKT、p-AKT和Ki67蛋白的阳性表达率较宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)各级的高,差异有统计学意义(P<0.05),鳞癌与腺癌无差别;CIN各级中PI3K、AKT和p-AKT的阳性表达率较正常宫颈组织高,差异有统计学意义(P<0.05),且这种差异随着CIN级别的递增而增大。(2)CIN各级宫颈组织中PI3K、AKT、p-AKT表达与Ki67表达有关。(3)PI3K和p-AKT表达与宫颈癌分化程度及临床分期明显相关,但与年龄、组织学类型和淋巴结转移无关。Ki67表达与各临床病理指标无明显相关性。结论:PI3K、AKT、P-AKT和Ki67的异常表达参与了宫颈癌的发生发展,与细胞增殖能力加强有关。Objective：To detect the expressions of PI3K, AKT, p-AKT, and Ki67 in cervical intraepithelial neoplasia （CIN） and cervical carcinoma and explore their relation with the occurrence and development of cervical diseases and the clinical significance. Methods：The expression of PI3K, AKT, p-AKT, and Ki67 in cervical intraepithelial neoplasia （CIN） and cervical carcinoma were detected by immunohistochemical SP methods. Results： （ 1 ） The expressions of PI3 K, AKT, p-AKT, and Ki67 protein were significantly higher in cervical carcinoma than CIN and normal cervical tissues. The difference was significant （ P 〈 0.05 ）. There was no significant difference between adenocarcima and squamous adenocarcinoma. CIN tissues had higher expressions of PI3K, AKT, p-AKT, and Ki67 proteins than normal control （ P 〈0.05 ） and the difference was increased with the elevated grade of CIN. （2） The expressions of PI3K, AKT, and p-AKT correlated with Ki67 in CIN tissues at various grades. （3） The expressions of PI3K and p-AKT had correlation with histological differentiation and clinical stage, but not with age, histological subtype, lymph node metastases. Expression of Ki67 was not associated with clinicopathological parameters. Conclusion：The aberrant expressions of PI3K, AKT, p-AKT, and Ki67 were involved in the occurrence and development of cervical cancer and related with the enhanced proliferation of tumor cells.

关 键 词：宫颈肿瘤 宫颈上皮内瘤样病变 免疫组织化学 

分 类 号：R730.2[医药卫生—肿瘤]