转化生长因子-β1反义寡核苷酸对人横纹肌肉瘤细胞RD增殖的影响  

Effects of Transforming Growth Factor-β1 ASON on the Proliferation of Human Rhabdomyosarcoma Cell Line RD

作　　者：王守立[1] 李世刚[1] 顾永平[1] 姚辉华[2] 秦正红[3] 

机构地区：[1]苏州大学医学院病理学教研室,苏苏州215123 [2]苏州大学附属第二医院普外科,江苏苏州215004 [3]苏州大学医学院药理学教研室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2007年第6期852-854,874,F0002,共5页Suzhou University Journal of Medical Science

基　　金：江苏省高校自然科学研究基金(Q2134605);江苏省资助经费招收博士后基金(51208)

摘　　要：目的研究转化生长因子(TGF)-β1反义寡核苷酸(ASON)对人胚胎性横纹肌肉瘤细胞系RD增殖的影响。方法采用反义寡核苷酸技术,以阳离子脂质体为载体,将人工合成的TGF-β1 ASON转染RD细胞,阻碍内源性TGF-β1/Smad信号的转导,以未转染的RD细胞组作对照。免疫荧光技术检测RD细胞TGF-β1蛋白表达水平;四甲基偶氮唑盐实验(MTT)检测RD细胞的增殖情况;流式细胞术(FCM)检测细胞周期。结果转染4 d后RD细胞TGF-β1蛋白表达量较转入2 d时明显减少,与对照组比较,差异有统计学意义(P<0.05);RD细胞活力明显降低,合成期(S期)细胞减少。结论TGF-β1 ASON可有效阻碍TGF-β1/Smad信号通路,在一定程度上可促进RD细胞退出细胞周期,并抑制RD细胞增殖。Objective To study the effects of transforming growth factor （TGF）-β1 antisense oligonucleotides（ASON） on proliferation of human rhabdomyosarcoma cell line RD. Methods Artificial- synthesized TGF-β1 ASON was transfected into RD cells by cation liposome vector to block endogenous TGF-β/Smad signal transduction, and RD cells without transfection served as the control group. The TGF-β1 protein expression of RD cells was inspected by immunofluorescent. The proliferation status of RD after TGF-β1 ASON-transfection was examined by MTT. The cell cycle was detected by FCM. Resuits The TGF-β1 protein expression of RD cells transferred for 4 days was decreased more than that of RD cells transferred for 2 days with significant difference （P 〈0.05） compared to the control group. The viability of RD cells in test group decreased obviously, and the S cells decreased. Conclusion The TGF-β1/Smad signal transduction pathway can be effectively blocked by the TGF-β1 ASON. TGF- β1 ASON could induce RD cells exit cell cycle, and inhibit the proliferation of RD cells.

关 键 词：转化生长因子Β1 横纹肌肉瘤 反义寡核苷酸技术 

分 类 号：R730.262[医药卫生—肿瘤]