当归中阿魏酸提取工艺的优化研究  

Ferulic acid in Angelica sinensis extracting technology

作　　者：金汝城[1] 李贵文[2] 马素丽[3] 周术涛[2] 赵国磊[2] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]兰州理工大学石油化工学院,甘肃兰州730050 [3]兰州理工大学理学院,甘肃兰州730050

出　　处：《中成药》2008年第4期516-519,共4页Chinese Traditional Patent Medicine

基　　金：甘肃省资助攻关项目(编号:2GS052-A43-008-04)

摘　　要：目的：测定当归中生物活性成分阿魏酸的含量并研究其提取工艺，提高活性成分的提取效率。方法：采用超声波辅助萃取法进行阿魏酸的提取，运用正交实验方法对影响阿魏酸提取的条件进行优化，同时采用高效液相色谱法进行分析检验。结果：阿魏酸在0．02244～0．1496μg范围内线性关系良好（r=0．9998），精密度RSD〈0．88％，加样回收率平均为96．8％，RSD为1．37％；得到了阿魏酸的最佳提取工艺，使阿魏酸的提取率达到了94．2％。结论：该工艺可充分将当归中的活性成分阿魏酸提取出来，提高了药材的利用率，AIM ： To determine the amount of bioactive component of ferulic acid in Angelica sinensis and study the extracting technology to develop the extracting rate. METHODS： Employing the technology of ultrasonic-assisted extraction and the method of orthogonal experiment to optimize the extracting technology of ferulic acid. At the same time HPLC was used in the detection. RESULTS ： The calibration curve was liner（r = 0. 999 8） in the range of 0. 022 44 -0. 149 6 μg for ferulic acid. Precision degree RSD 〈0.88%. The average recovery was 96.8% and RSD 1.37%. The best extracting technology was got and the extracting rate reached 94.2%. CONCLUSION： With the technology we can extract ferulic acid sufficiently and raise the using rate of medicinal materials.

关 键 词：当归 阿魏酸 超声波辅助萃取 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]