小鼠皮肤切创黏着斑激酶和磷酸化黏着斑激酶的表达及其变化规律  

Expressions and Time-dependent Changes of FAK and phospho-FAK during Skin Incised Wound Healing in Mouse

作　　者：杨大利[1] 官大威[1] 张建军[1] 

机构地区：[1]中国医科大学法医学院,辽宁沈阳110001

出　　处：《法医学杂志》2008年第2期81-84,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30271347);教育部留学归国基金资助项目(教外留司[2000]367号)

摘　　要：目的研究小鼠皮肤切创愈合过程中,黏着斑激酶(focal adhesion kinase,FAK)和磷酸化黏着斑激酶(phospho-FAK,p-FAK)在损伤区及损伤周边区内的表达及其变化规律。方法应用免疫组织化学染色技术和免疫印迹(Western blot)方法观察小鼠背部切创后损伤区及损伤周边区FAK及p-FAK的表达情况。结果伤后3h,FAK和p-FAK主要表达于多核粒细胞;伤后6～24h,主要表达于大部分浸润的多核粒细胞和单核细胞;伤后3～14d,主要表达于单核细胞和成纤维细胞。伤后FAK的阳性细胞率逐渐增高,于3d达到高峰,随后迅速下降;伤后p-FAK的阳性细胞率也逐渐增加,于12h达到高峰,之后逐渐下降。Western blot结果显示,正常皮肤中FAK及p-FAK均有表达。FAK表达强度变化不是十分明显,但仍可见在3d时含量达到最高。p-FAK蛋白含量伤后逐渐增加,12h表达最强,随后逐渐下降。结论在小鼠皮肤切创愈合过程中,FAK和p-FAK阳性细胞率呈时间规律性变化,可用于皮肤切创损伤时间的推断。p-FAK呈现出明显的时间规律性变化,说明作为皮肤损伤时间的推断指标要优于FAK。Objective To study the expressions of focal adhesion kinase（FAK） and phospho-FAK（p-FAK） during skin incised wound healing and the applicability of time-dependent expressions of FAK and p-FAK. Methods The expression of FAK and p-FAK in cutaneous incised wound in mouse were investigated by immunohis- tochmeistry and Western blotting. Results FAK and p-FAK expression were detected in polymorphonuclear cells （PMNs） in the wound and adjacent regions 3 hours post-injury. The expressions of FAK and p-FAK were detected in a large number of infiltrating PMNs and some of mononuclear cells 0VINCs） from 6 to 24 hours after injury. The MNCs and fibroblastic cells （FBCs） accounted for most part of the FAK and p-FAK positive cells from 3 to 14 days after injury. The numbers of FAK-positive cells increased continuously, reaching a peak at post-injury day 3, and then started to decrease from post-injury day 5 to 14. The changes of p-FAK-positive cells were similar to that of the FAKs, and reached a peak at 12 hours after injury. Conclusion Both FAK and p-FAK displayed a time-dependent expression during skin incised wound healing in mouse, with p-FAK being superior to FAK. Both FAK and p-FAK may potentially be used as new markers for determination of the wound interval.

关 键 词：免疫组织化学 黏着斑激酶 磷酸化黏着斑激酶 皮肤损伤 损伤时间 免疫印迹 

分 类 号：R735.35[医药卫生—肿瘤] R394[医药卫生—临床医学]