基于NMR的代谢组学方法对硝酸钕急性生物效应的研究  

Studies on the Acute Biochemical Effects of Nd(NO_3)_3 by Nuclear Magnetic Resonance-based Metabonomics

作　　者：廖沛球[1] 张晓宇[2] 魏来[1] 李伟生[1] 吴亦洁[1] 李晓晶[1] 倪嘉缵[1] 裴奉奎[1] 

机构地区：[1]中国科学院长春应用化学研究所 [2]燕山大学环境与化学工程学院生物工程系,秦皇岛066004

出　　处：《分析化学》2008年第4期426-432,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20575065,20637010)

摘　　要：采用现代核磁共振和模式识别技术,分析了腹腔注射给药2、10和50mg/kg体重剂量硝酸钕48h内Wistar大鼠尿液和血清的核磁共振氢谱。由尿液及血清中内源性代谢物如柠檬酸、肌酸酐、N-氧三甲胺、氨基酸、乳酸、琥珀酸、牛磺酸及葡萄糖等物种的浓度变化,结合大鼠血清指标和肝、肾组织切片图研究了轻稀土化合物Nd(NO3)3在大鼠体内的急性生物效应。结果表明,3个剂量的Nd(NO3)3主要对大鼠肝脏造成了不同程度的损伤,而且随着剂量的升高渐趋严重。同时,Nd(NO3)3也对肾脏的特定部位(肾乳头、肾小管)造成了损害。The biochemical effects of Nd （ NO3 ） 3 was studied by NMR-based metabonomics. Male Wistar rats were administrated intraperitoneally with Nd（ NO3 ）3 at doses of 2, 10 and 50 mg/kg body weight. Urine was collected over a 48-h period after dosing and serum was collected after 48 h. ^1H NMR spectra of biofluids （urine and serum） were analyzed by pattern recognition using principal components analysis. The studies showed that there was a dose-related biochemical effects of Nd（ NO3 ）3 treatment. The liver and kidney （renal tubular and renal papilla） damage induced by Nd（ NO3 ）3 was characterized by the variations of many endoge- nous metabolites, such as citrate, creatinine, N-trimethyl dioxide, dimethyl amine, dimethyl glycine, lactate, taurine and glucose. The NMR-based metabonomics can be applied to study the biochemical effects or toxicities of other compounds.

关 键 词：硝酸钕 体液 核磁共振 生物效应 模式识别 代谢组学 

分 类 号：R96[医药卫生—药理学]