脑胶质瘤中P170、TOPⅡ表达与其增殖活性关系的研究  

Correlation of Expressions of P170,TOPⅡ and Proliferation in Gliomas

作　　者：孙淑清[1] 李桂林[1] 苏玉金[1] 徐作霖[1] 崔云[1] 方静宜[1] 李敬军[1] 

机构地区：[1]北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2008年第4期349-351,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的探讨人脑胶质瘤组织中多药耐药基因（P170）、拓扑异构酶Ⅱ（TOPⅡ）的表达与其增殖活性的相关性。方法以核抗原（Ki-67）作为增殖指标,应用单克隆抗体、免疫组化技术S-P法观察54例低级别（Ⅰ～Ⅱ级）和54例高级别（Ⅲ～Ⅳ级）胶质瘤组织中P170、TOPⅡ和Ki-67的表达,并对临床资料进行随访及分析。结果P170、TOPⅡ和Ki-67蛋白在低级别和高级别胶质瘤之间的表达差异均有非常显著性意义（P〈0.01）;相关分析显示,在胶质瘤中,P170与Ki-67的表达呈负相关（r=-0.276,P=0.019）,TOPⅡ与Ki-67的表达呈显著正相关（r=0.637,P〈0.001）,P170与TOPⅡ的表达之间无相关性。结论胶质瘤中P170、TOPⅡ的表达与其恶性进展相关。Objective To investigate the correlation of the expressions of P170, topoisomerase Ⅱ （TOP Ⅱ ） and the proliferation in gliomas. Methods Ki 67 was used as the marker of the proliferation in gliomas. The expressions of P170, TOPⅡ and Ki 67 were tested by S-P immunohistochemical technique using monoclonal antibody to their protein in 54 cases of low-grade gliomas （WHO Ⅰ Ⅱ grade） and 54 cases of high grade gliomas （WHO Ⅲ-Ⅳ grade）, analysis of the clinical and follow-up were performed. Results The expressions of P170, TOP Ⅱ and Ki-67 had significant differences between low-grade and high grade gliomas （P 〈0.01）. Correlation analysis showed that the expression of P170 was negatively related with Ki-67 （ r =0. 276, P = 0. 019） ; the expression of TOP Ⅱ was positively related with Ki 67 （ r = 0. 637, P 〈0. 001）; there was no correlation between the expressions of P170 and TOP Ⅱ. Conclusion The expressions of P170 and TOP Ⅱ are associated with the malignant prognosis in gliomas.

关 键 词：胶质细胞瘤 P170(多药耐药基因) TOPⅡ(拓扑异构酶) Ki-67(核抗原) 

分 类 号：R739.41[医药卫生—肿瘤]