扇贝裙边糖胺聚糖拮抗高糖对血管内皮细胞损伤机理的研究  

Antagonism effect of scallop skirt glycosaminoglycan on damage of vascular endothelial cell by highly concentrated glucose

作　　者：王瑞鑫[1] 王春波[2] 罗世滨[1] 李裕强[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209 [2]青岛大学医学院

出　　处：《山东医药》2008年第11期21-23,共3页Shandong Medical Journal

基　　金：山东大学威海分校大学生科技创新项目(A07053)

摘　　要：目的探讨扇贝裙边糖胺聚糖(SS-GAG)拮抗血管内皮细胞损伤性疾病的价值。方法取体外培养生长良好的血管内皮细胞(ECV304),建立高糖诱导的细胞损伤模型。在高浓度葡萄糖(55.5 mmol/L)状态下加入不同浓度SS-GAG,继续培养至12、24、36、48 h,倒置显微镜下观察细胞形态,用3H-TdR掺入法检测细胞增殖活性。结果①高浓度葡萄糖使ECV304细胞形态发生改变,细胞增殖活性受到明显抑制,且呈浓度和时间依赖性;②SS-GAG与高浓度葡萄糖共同作用下,内皮细胞形态及增殖活性基本正常,此效果与SS-GAG浓度呈正比。结论高浓度葡萄糖对血管内皮细胞具有明显损伤作用;SS-GAG可减轻高浓度葡萄糖对血管内皮细胞的损伤,保护血管内皮细胞;SS-GAG对防治心脑血管疾病和糖尿病血管病变及动脉粥样硬化的发生、发展有积极意义。Objective To explore antagonism the effect of scallop skirt glycosaminoglycan （SS-GAG） on damage of vascular endothelial cell by highly concentrated glucose and its mechanism. Methods The vascular endothelial cell ECV304 was cultured in vitro, and the damage model of endothelial cells was established by highly concentrated glucose. Under the condition of highly concentrated glucose （55.5 mmol/L）, different concentration SS-GAG was added into the cells, and the cell morphology was observed after cultured for 12 h,24 h, 36 h and 48 h respectively. Cell proliferation activity was measured by the method 3^H-TdR. Results The cell morphology was changed by highly concentrated glucose. Cell proliferation activity was depressed, and the longer the time, the damage much heavy under the higher concentration of glucose（55. 5 mmol/L） （P 〈0. 01 ）. However, SS-GAG weakened the damaged effect of highly concentrated glucose, and made the cell morphology, proliferation activity recovered to normal level. The protective effect was higher under the higher concentration of SS-GAG, and there was no difference between these recovered cells and normal control, but big difference occurred between these cell and positive cell control in highly concentrate glucose. Conclusions Highly concentrate glucose can obvious damag vascular endothelial cell, which may induce cell morphology changed. SS-GAG possess obvious protective effect on vascular endothelial cell damaged by highly concentrated glucose. SS-GAG may have important effect on preventing and curing cardiovascular disease, diabetes and atherosclerosis.

关 键 词：扇贝 糖胺聚糖 葡萄糖溶液 高渗 血管内皮细胞 

分 类 号：R587.1[医药卫生—内分泌]