淋巴滞留性脑病大鼠脑组织超微结构及Bax、Bcl-2表达的变化  

Changes of ultrastructure and Bax,Bcl-2 expression of rat brain in lymphostatic encephalopathy

作　　者：李育娴[1] 张军[2] 石红军[2] 夏作理[3] 陈连璧[4] 

机构地区：[1]同济大学医学院生理学教研室,上海200092 [2]同济大学医学院生物学教研室,上海200092 [3]泰山医学院 [4]山东大学医学院

出　　处：《临床神经病学杂志》2008年第2期113-115,共3页Journal of Clinical Neurology

基　　金：同济大学医科发展基金资助项目(1509219030)

摘　　要：目的研究淋巴滞留性脑病(LE)脑组织超微结构及大脑皮质Bax、Bcl-2表达的变化。方法用阻断颈部淋巴引流的方法制作LE大鼠模型,观察术后1d、2d、3d、5d、7d和14d脑组织超微结构的变化;免疫印迹法检测大脑皮质Bax和Bcl-2蛋白的表达;并与正常对照组比较。结果电镜下见LE组大鼠脑组织有水肿,血管周围出现半月形间隙、细胞肿胀,可见神经细胞凋亡和坏死,以LE5d组最明显。LE3d、5d、7d组大脑皮质Bax和Bcl-2蛋白的表达明显高于正常对照组(均P<0.01),LE5d组为最高;Bax/Bcl-2的变化与此相同。结论LE大鼠出现脑部水肿及神经细胞凋亡和坏死,大脑皮质Bax和Bcl-2表达增高。Objective To investigate the changes of uhrastructure and Bax, Bcl-2 expression of cerebral cortex in lymphostatic encephalopathy （LE）. Methods LE models were established by occluding and removing both the shallow and deep cervical lymph nodes in rats. Uhrastructural changes of the cerebral cortex were observed at 1 d, 2 d, 3 d, 5 d, 7 d and 14 d after operation. Expression of Bax and Bcl-2 were examined by Western blot. Results Brain edema appeared under electron microscope in LE model group. The perivascular spaces in the cortex were enlarged and full of fluid, the dropsical nerve fibers had swelling and fragmented myelin sheath. Some neurons showed apoptotic conformation and some had characters of necrosis. The changes mentioned above were the most serious at day 5. The protein expression of Bax, Bcl-2 and the ratios of Bax/Bcl-2 in LE 3 d,5 d,7 d groups were significantly higher than those in normal control group （ all P 〈0. 01 ）. All of them reached a peak at 5 d. Conclusion Brain edema, apoptosis and necrosis are appeared, the expression of Bax and BcI-2 are increased in LE models of rat.

关 键 词：淋巴滞留性脑病 BAX BCL-2 

分 类 号：R741[医药卫生—神经病学与精神病学]