可卡因-苯丙胺调节转录肽对缺血性脑损伤及其神经元半胱天冬氨酸蛋白酶活性的影响  

Effects of Cocain and amphetamine-regulated transcript peptides on ischemic brain damage and Caspase-3 activity of neurons

作　　者：陈雪梅[1] 贾佳[2] 徐运[1] 

机构地区：[1]南京大学医学院附属鼓楼医院神经内科,210008 [2]南京大学医药生物技术国家重点实验室

出　　处：《临床神经病学杂志》2008年第2期136-138,共3页Journal of Clinical Neurology

基　　金：国家自然科学基金资助项目(30470612)

摘　　要：目的探讨可卡因-苯丙胺调节转录(CART)肽对缺血性脑损伤及其半胱天冬氨酸蛋白酶(Caspase)活性的影响。方法将12只小鼠随机分为CART组与对照组,CART组小鼠侧脑室注射CART多肽,对照组注射生理盐水,然后制作脑缺血模型,2,3,5-氯化三苯基四氮唑(TTC)染色测量梗死体积。体外培养小鼠皮质神经元,制作缺糖缺氧神经元模型,加入CART多肽或生理盐水,用噻唑蓝染色测细胞生存率,酶联法检测Caspase-3活性。结果CART组脑梗死体积[(0.225±0.044)mm3]显著小于对照组[(0.389±0.055)mm3](P<0.05);CART组神经元生存率(0.494±0.056)显著高于对照组(0.348±0.174)(P<0.05);CART组神经元Caspase-3活性(45674±9780)显著低于对照组(51172±2776)(P<0.01)。结论CART多肽可明显减少缺血性脑梗死体积,提高缺氧神经元的生存率,并降低缺氧神经元的Caspase-3活性。Objective To investigate effects of Cocain and amphetamine-regulated transcript （CART） peptides on cerebral ischemic damage and Caspases-3 activities of neurons. Methods 12 mice were randomly divided into CART group and the control group. Mice model of cerebral ischemic was made, after CART group was lateral ventricle injected CART peptides, control group was lateral ventricle injected saline water. Infarction volume was detected by TTC staining. Viability rate of OGD-neurons with CART peptides or salinewater, after mice cortical neuron in vitro was detected by MTT. Caspases-3 activitie was measured by ELISA. Results Infarction volume in the CART group [ （0. 225 ± 0. 044 ） mm^3 ] was significantly smaller than that in the control group [ （ 0. 389 ± 0. 055 ） mm3 ] （ P 〈 0. 05）. Viability rate of OGD-neurons in the CART group（0. 494 ± 0. 056） was significantly higher than that in the control group （0. 348 ± 0. 174, P 〈 0.05）. Caspase-3 activitie of OGD-neurons with CART peptides （45674 ± 978 ） was lower significantly than that with control group （ 51172 ± 2776） （ P 〈 0. 05）. Conclusion CART peptides can significantly reduce infarction volume of ischemic cerebral infarction, enhance viability of OGD-neurons and suppress Caspases-3 activities in OGD-neurons.

关 键 词：可卡因-苯丙胺调节转录肽 脑缺血 半胱天冬氨酸蛋白酶-3 

分 类 号：R743.3[医药卫生—神经病学与精神病学]