原癌基因Fra-1高表达与乳腺癌细胞转移表型的关系  

Association of overexpression of proto-oncogene Fra-1 with the metastatic phenotype of breast cancer cells

作　　者：宋玉华[1] 宋三泰[2] 江泽飞[2] 钱露[3] 陈立勇[3] 郭宁[3] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266031 [2]军事医学科学院附属医院,北京100071 [3]军事医学科学院基础医学研究所,北京100850

出　　处：《西安交通大学学报（医学版）》2008年第2期171-175,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的研究原癌基因Fra-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系。方法提取乳腺癌细胞系MDA231细胞的总RNA,通过RT-PCR扩增Fra-1全长基因,将其克隆入pcDNA3.1(-)myc-his表达载体;应用脂质体法转染MCF-7细胞,观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响。结果高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力。结论原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移,在肿瘤细胞的侵袭和转移过程中发挥作用。Objective To illustrate the association of Fra-1 overexpression with motility and invasiveness of breast cancer cells. Methods Fra-1 gene was obtained by RT-PCR. PCR products were cloned into the plasmid pcDNA3.1（-） myc-his to create the recombinant plasmid pcDNA3.1 （-） myc-his/Fra-1. The plasmid was then transfected into human breast cancer cell line MCF-7. The proliferation, adhesion and migration of MCF-7 cells stably overexpressing Fra-1 were investigated. Results The enhanced growth, adhesion and migration of MCF-7 cells overexpressing Fra-1 were observed. Conclusion Invasiveness and metastasis of tumor cells could be promoted by Fra-1 mediated enhancement of adhesion and migration of tumor cells.

关 键 词：乳腺癌 Fra-1 AP-1 肿瘤转移 重组质粒 转染 

分 类 号：R735.1[医药卫生—肿瘤]