膀胱癌组织中端粒酶活性研究  

Study of telomerase activity in bladder cancer

作　　者：彭钧铮[1,2] 张元芳[1,2] 丁强[1,2] 潘崇贤 

机构地区：[1]上海医科大学泌尿外科研究所 [2]美国芝加哥洛约那大学医学和癌症研究中心

出　　处：《中华泌尿外科杂志》1997年第10期601-603,共3页Chinese Journal of Urology

摘　　要：应用聚合酶链反应（ＰＣＲ）为基础的端粒酶活性检测方法（ＴＲＡＰ法），对５例正常膀胱组织、４例膀胱炎症组织、４２例膀胱癌组织和癌旁正常组织进行检测。正常膀胱组织和膀胱炎症组织无端粒酶活性，膀胱癌组织端粒酶表达阳性率７６．２％，癌旁组织阳性率为２６．２％，端粒酶活化与膀胱癌分期分级呈正相关。结果提示：端粒酶活化与膀胱癌浸润发展密切相关，癌旁组织端粒酶活化提示肿瘤微浸润可能。Using a polymerase chain reaction based telomerase activity assay (TRAP),we examined telomerase activity in 5 normal bladder tissues, 4 bladder infection tissues, 42 bladder cancer tissues and 42 adjacent noncancerous bladder tissues. None of the normal and inflammatory bladder tissue exhibited telomerase activity. 32 of the 42(76.19%) bladder cancer tissues revealed telomerase activity. In the adjacent normal tissue taken from the bladder cancer, 11 of the 42(26.19%) were telomerase positive. The telomerase is more frequently activated in advance ,not well differentiation bladder tumors. The results indicate: telomerase activation has strong relationship with the invasion and development of bladder cancer. The telomerase activation of the paracancer tissue shows the possibility of microinvasion.

关 键 词：膀胱肿瘤 端粒酶 PCR 

分 类 号：R737.140.2[医药卫生—肿瘤]