X线和苏拉明联合应用诱导肾癌GRC-1细胞凋亡的研究  

An experimental study of cell apoptosis in GRC 1 cell line induced by the combined use of X ray and suramine

作　　者：杨宝龙[1,2,3,4] 顾方六[1,2,3,4] 丁义[1,2,3,4] 赵小东 刘德祥 俞莉章 

机构地区：[1]北京医科大学第一医院 [2]北京医科大学泌尿外科研究所 [3]国家计划生育研究所分子生物实验室 [4]大连市中心医院泌尿外科

出　　处：《中华泌尿外科杂志》1997年第9期518-521,共4页Chinese Journal of Urology

摘　　要：为寻求敏感而有效的治疗肾癌的方法，采用Ｘ线照射和不同剂量的苏拉明联合应用的方法诱导肾癌ＧＲＣ１细胞凋亡，以原位缺口末端标记和ＤＮＡ片断梯度电泳分析法检测。结果显示：本法可诱导肾癌ＧＲＣ１细胞凋亡，并随苏拉明剂量的增加，凋亡细胞增多（最高６８．２％）。随时间延长凋亡细胞数增多（最高达６６．２％）。ＤＮＡ片断梯度电泳分析表明，随苏拉明剂量增加和给药后时间的延长，ＤＮＡ片断梯度改变更加明显。结果提示Ｘ线和苏拉明联合应用治疗肾癌，可望取得良好的治疗效果。RCC cell line, GRC 1, was treated with X ray and different doses of suramine and cell apoptosis was serially detected by means of TUNEL (TdT mediated dUTP biotin nick end labelling). Apoptosis of GRC 1 has been induced by radiochemotherapy with the combined use of X ray and suramine. The amount of apoptostic cells was positively related to suramine dosage and the duration elapsed. DNA fragment electro phoresis also showed apparent changes. It was suggested that RCC might be effectively treated by radiochemotherapy with the combined use of X ray and suramine.

关 键 词：肾肿瘤 细胞凋亡 X线 苏拉明 治疗 

分 类 号：R737.110.5[医药卫生—肿瘤]