浅低温对犬全脑缺血再灌注后脑组织诱导型热休克蛋白70表达的影响  

Effect of mild hypothermia on the expression of the inducible HSP70 in canine brain tissue after globai cerebral ischemia reperfusion

作　　者：李锋华[1] 谭秀娟[2] 蔡宏伟[2] 程志刚 郭曲练[2] 

机构地区：[1]湖南医科大学湘雅医院麻醉,长沙市410008 [2]湖南医科大学湘雅医院麻醉,长沙市410008

出　　处：《中华麻醉学杂志》1997年第10期603-605,共3页Chinese Journal of Anesthesiology

摘　　要：目的:研究犬全脑缺血再灌时浅低温对热休克蛋白70(HSP70)表达的影响,探讨热休克蛋白在低温脑复苏中的作用。方法:采用犬心脏停跳再复苏模型。14只犬随机分成三组:非缺血对照组(n=4)、常规治疗组(n=5)和浅低温组(n=5)。用免疫组化测定脑组织内HSP70表达阳性细胞数密度和反应产物灰度值。结果:犬全脑缺血再灌后HSP70表达明显增强(P<0.01)。浅低温组HSP70表达增强比常规治疗组更明显(P<0.01)。病理损伤明显减轻。结论:浅低温能增加再灌脑组织HSP70表达,并可能是低温脑复苏机制之一。Objective:To study the mechnism of mild hypothermia during cerebral resuscitation. Method:Fourteen dogs were anaesthetized with ketamine and fentanyl and were divided randomly into three groups. Four dogs as nonischemic control(group A).recived general care for 24 hours after operation without cardiac arrest. The other ten dogs underwant a 18-minute cardiac arrest.were resuscitated with standard CPR.and supported by intensive care for 24 hours,then divided into two groups. Five dogs were treated with routine cerebral resuscitation measures (RCRM) (group B):five dogs were treated with RCRM and mild hypothermia started 10 min after CPR and mantained at 34 C±0.5 C for 12 hours (group C). At the end of experiment,the parietal cerebral cortex was obtained to detect the expression of heat shock proteins with immunohistochemical technique and to observe the change of histomorphology. Result: The pathologic change in group C was more obviously mitigated than that in group B. The HSP70-LI positive neuronal density and grey values in group B and C were more significantly increased than those in group A (P<0.01 );The HSP70-LI positive neuronal density and grey values in group C was more significantly than that in group B (P<0.01 ). Conclusion: The mild hypothermia might alleviate the histopathologic damage of brain and increase HSP70 expression after global cerebral ischemia reperfusion injury.

关 键 词：再灌注损伤 热休克蛋白 脑缺血 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]