水稻籼粳交DH群体的构建及其基因型偏态分离分析  

Construction and Distorted-segregation Analysis in Genotype of DH Population from indica -japonica Cross

作　　者：季芝娟[1] 叶胜海[1] 马良勇[1] 李西明[1] 王晓光[1] 蔡晶[1] 别文力[2] 杨长登[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [2]长江大学农学院,荆州430025

出　　处：《农业生物技术学报》2008年第2期315-319,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2006AA10Z1B5);浙江省自然科学基金(No.Y305160);中国水稻研究所青年创新基金资助

摘　　要：对籼(Oryza sativa L.ssp.indica)粳(O.sativa L.ssp.japonica)杂交组合中花11(粳)/中组14(籼)F1进行花药培养,共获得112个加倍单倍体(DH)再生植株。利用亲本间多态性SSR分子标记对该DH群体再生植株进行纯合性鉴定,并分析该群体的基因型偏离情况。选用22个多态性SSR标记分析的结果表明,所得再生植株都来自花粉母细胞的纯合植株,不存在来自体细胞的杂合株,确保了DH群体的准确性。带型分析结果显示,该群体未发生异常的基因型偏态分离。因此在组织培养体系中引进SSR标记,可以快速而准确地构建DH群体,基因型的偏态分离分析保证了群体的质量。F1 anther of Zhonghua 11/Zhongzu 14 （japonica/indica）combination was cultured and one hundred and twelve double haploid （DH） regenerant plants were obtained. All of the DH plants were analysed for their homozygosity using 22 polymorphic SSR markers between the two parents. Distorted segregation analysis in genotype for the population was carried out. Polymorphic SSR analysis suggested that the regenerant plants were all originated from microspore and no heterozygots from somatic cells. Band pattern analysis with polymorphic SSRs showed that there was no abnormal distribution in genotype for the population. The results indicated that the DH population consisted accurately of microspore-originated regenerants and quality of the population was guaranteed via distorted segregation analysis in genotype with SSR markers.

关 键 词：水稻 DH群体 SSR标记 纯合 基因型 偏态分离 

分 类 号：S188[农业科学—农业基础科学]