TACE在小鼠动情周期的表达规律  

Expression pattern of TACE in mouse endometrium during the estrus cycle

作　　者：杨洁玉[1] 李彤 杨戎[1] 谢怡[1] 魏莎莉[1] 郗强[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生理教研室 [2]重庆市人口与计划生育委员会,重庆400016

出　　处：《重庆医科大学学报》2008年第4期416-418,440,共4页Journal of Chongqing Medical University

基　　金：重庆市科委自然科学基金项目(CSTC,2007BB5273);重庆市计划生育科研项目(2007-1-8)

摘　　要：目的:初步探讨肿瘤坏死因子α转换酶(Tumor necrosis factor-αconverting enzyme,TACE)在小鼠动情周期中的表达规律。方法:用RT-PCR及免疫组化方法分别检测动情周期各期小鼠子宫内膜TACEmRNA和蛋白的表达。结果:TACEmRNA在动情周期小鼠子宫内膜呈规律性表达,在动情期表达明显增强,与其它3期相比有显著性差异(P﹤0.01);其它3期间无显著性差异(P>0.05)。免疫组化分析显示TACE在子宫内膜的表达规律与RT-PCR一致。结论:TACE通过裂解EGFR家族多种配体释放其胞外区,从而激活EGFR信号途径,在子宫内膜的周期性变化中起着重要作用。Objective：To primarily investigate the expression pattern of tumor necrosis factor-α converting enzyme（TACE） in mouse endometrium during the estrus cycle. Methods：Reverse transcription polymerase chain reaction（RT-PCR ） and immunohistochemistry were used to detect the expression of TACE mRNA and protein in mouse endometrium during each stage of the estrus cycle. Results：The expression of TACE mRNA in mouse endometrium during estrus cycle had regular pattern. The TACE mRNA/β-actin mRNA expression in estrus stage was higher than those of the other 3 groups,and there was significant difference between estrus stage and the other 3 groups（P〈 0.01）. Besides,there was no significant difference among the other 3 groups（P〉0.05）. Immunohistochemical analysis showed the same expression pattern of TACE in endometrium as RT-PCR. Conclusion：TACE may play a key role in cyclic changes of normal endometrium through proteolytic removal of the extracellular domain of multiple ligands of EGFR family to release their extracellular domains which is capable of activating EGFR signal way.

关 键 词：TACE 子宫内膜 动情周期 RT—PCR 免疫组化 

分 类 号：R394[医药卫生—医学遗传学]