细胞因子对K562细胞HSP gp96基因及蛋白表达的影响  

Effects of Cytokines on the Gene and Protein Expressions of Heat Shock Protein gp96 in K562 Cells

作　　者：王同国[1] 史斌[1] 王昕[1] 王立茹[1] 陈以娟[1] 

机构地区：[1]首都医科大学附属复兴医院血液科

出　　处：《首都医科大学学报》2008年第2期128-132,共5页Journal of Capital Medical University

基　　金：首都医学发展科研基金(2003-086)资助项目~~

摘　　要：目的研究细胞因子与K562细胞共孵育时不同浓度与不同时间条件对gp96基因及蛋白的影响。方法分别将浓度为0kU/L、200kU/L、400kU/L、600kU/L、800kU/L、1000kU/L的IFN-α及0μg/L、2μg/L、4μg/L、6μg/L、8μg/L、10μg/L的IFN-γ与K562细胞共孵育12h,用Western blot及RT-PCR法分析其中的gp96 mRNA和蛋白。分别将浓度为600kU/L的IFN-α和6μg/L的IFN-γ与K562细胞共孵育0、3、6、9、12、18、24、36h检测gp96 mRNA含量。结果1)不同浓度IFN-α和IFN-γ分别与K562细胞共孵育12h后,hsp gp96基因及其蛋白的表达出现相似的改变,即随着浓度的增加表达量逐步增加,当浓度达IFN-α600kU/L、IFN-γ6μg/L后,表达量逐步下降。2)IFN-α 600k U/L、IFN-γ6μg/L分别与K562细胞共孵育,细胞中hsp gp96 mRNA表达量均先增加后逐步下降,在9h达高峰。结论IFN-α、IFN-γ与K562细胞共同孵育,能通过上调细胞中hsp gp96 mRNA增加HSP GP96蛋白。Objective Tumor-derived heat shock protein glycoprotein 96 （HSP GP96 ） can be used as vaccines for refractory cancers. The effects of eytokines on the gene and protein expressions of GP96 in K562 cells at different concentrations and different hours were investigated. Methods K562 cells were eoineubated with different concentrations of eytokines for 12 hours. GP96 mRNA and GP96 proteins of K562 cells were analyzed. Total RNA of K562 cells, which were respectively eoineubated with concentrations increases, but they descend at certain concentrations（ IFN-α 600 kU/L or IFN-γ6 μg/L at different hours, were analyzed. Results 1 ） Dose-effect： changes of the gene and protein expressions of GP96 from K562 cells which were respectively eoineubated with different concentrations of IFN-α and IFN-γ for 12 hours were similar： they gradually increased with the concentrations, but descended at certain concentrations （ IFN-α 600, kU/L, IFN-γ 6 μg/L）. 2） Time-effect ： steady level of GP96 mRNA were achieved up to 9 hours of continuous exposure to IFN-α 600 kU/L and IFN-γ 6 μg/L, and then descended gradually. Conclusion IFN-α and IFN-γcan increase the protein expressions of GP96 from K562 cells by increasing gp96 gene expression.

关 键 词：干扰素Α 干扰素Γ 热休克蛋白GP96 

分 类 号：R733.72[医药卫生—肿瘤]