人神经干细胞移植到缺氧缺血性脑损伤新生大鼠脑内的存活及分布  

Survival and distribution of human neural stem cells transplanted into cerebral ventricles of neonatal rats with hypoxic-ischemic brain damage

作　　者：王桂芬[1] 栾佐[2] 高宝勤[1] 尹国才[2] 白洁[3] 屈素清[2] 

机构地区：[1]首都医科大学附属天坛医院儿科,北京市100050 [2]解放军海军总医院儿科,北京市100037 [3]北京医科大学附属第一医院儿科,北京市100034

出　　处：《中国组织工程研究与临床康复》2008年第12期2201-2205,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的：新生儿缺氧缺血性脑病是导致脑性瘫痪的重要原因,至今缺乏有效疗法。将体外培养的人神经干细胞经脑室移植入缺氧缺血性脑损伤新生鼠,观察植入细胞在宿主脑内的存活、迁移及分化。方法：实验于2005-01/09在解放军海军总医院儿科实验室完成。①对象：神经干细胞来源于孕12周流产的人胎儿脑组织,孕妇签署知情同意书,符合医院伦理委员会规定。清洁级SD新生鼠80只,随机数字表法分为细胞移植组、模型对照组,40只/组,实验过程中对动物的处置符合动物伦理学标准。②实验方法：取人胚胎脑组织,机械分散法分离单个核细胞,接种于添加表皮生长因子、碱性成纤维细胞生长因子、白血病抑制因子的N2培养基中,获取生长旺盛的人神经干细胞球,制成单细胞悬液,浓度约为5.0×10^11 L^-1,培养6d后行PKH标记用于植入后示踪。两组新生鼠均建立缺氧缺血性脑损伤模型,造模后3d,细胞移植组损伤侧脑室缓慢注入5μL人神经干细胞悬液,模型对照组于相同部位注入等量生理盐水。③实验评估：取未经PKH标记的细胞球,通过免疫细胞化学染色鉴定巢蛋白的表达及其向神经元、星形胶质细胞的分化情况。分别于细胞移植后1,2,4周及3个月,常规取脑组织,行免疫组织化学和荧光分析,观察植入后细胞存活及分布情况。结果：在造模及细胞移植过程中,因麻醉、出血细胞移植组新生鼠死亡5只,模型对照组死亡7只,存活率85%～90%。①神经干细胞的鉴定及分化：80%活细胞巢蛋白呈阳性表达,并可分化为神经元、星形胶质细胞。②神经干细胞植入后存活及分布情况：植入后1周,神经丝蛋白阳性细胞多位于损伤侧皮质及海马处,纹状体、脑干、小脑、嗅球也有少量分布。植入后2周,海马和皮质可见神经丝蛋白阳性细胞,胞体伸出的神经微丝更长,细胞数量与1周时基本相似。植入�AIM： Neonatal hypoxic-ischemic encephalopathy （HIE） is an important cause of cerebral palsy. There is no effective therapy for HIE. This study investigated the survival, migration and differentiation of in vitro cultured human neural stem cells （NSCs） following transplantation into the cerebral ventricles of neonatal rats with hypoxic-ischemic brain damage （HIBD）. METHODS： Experiments were performed at the Laboratory of Department of Paediatrics of Navy General Hospital of Chinese PLA from January to September 2005. （1）NSCs were harvested from the brain of a 12-week old abored human fetus. The pregnant woman signed an informed consent. Experiments were accorded with the rules of Hospital＇s Ethics Committee. Eighty Spragne-Dawley neonatal rats were equally divided into a NSC transplantation group and a model control group by the random digits table method. Animal interventions in the experiment were accorded with the Animal Ethical Standards. （2）Mononuclear cells were collected from human fetal brain tissues by mechanical suspension dispersion and inoculated in the N2 medium containing epidermal growth factor （EGF）, basic fibroblast growth factor （bFGF） and leukemia inhibitory factor （LIF）. Human NSC spheres were prepared into single cell suspension at approximately 5.0×10^11 L^-1. After 6-days culture, NSCs were labeled with PKH. Neonatal rat models of HIBD were established in both groups. Three days later, 5 μL human NSC suspension was injected into the cerebral ventricle in the NSC transplantation group, and 5 μL saline was injected into the same region in the model control group. （3）Nestin expression and differentiation into neurons and astrocytes in human NSC spheres labeled without PKH were identified by immunocytochemistry. At 1, 2, 4 weeks and 3 months after transplantation, the brains of rats were collected and examined by immunohistochemistry and analysis to determine the survival and distribution of NSCs .RESULTS： Five neonatal rats in the NSC transplantati

关 键 词：神经干细胞 移植 缺氧缺血 新生鼠 

分 类 号：R394.2[医药卫生—医学遗传学]