SHIV-B'whu感染中国猕猴的组织病理学观察  

Pathological Observation on Chinese Rhesus Monkeys with Recombined Simian/Human Immunodeficiency Virus

作　　者：唐志佼[1] 代明[1] 包容[1] 王勇[1] 张晶[1] 鲜巧阳[1] 饶艳[1] 李任翔[1] 孙理华[1] 

机构地区：[1]武汉大学ABSL-3实验室/动物实验中心,武汉430071

出　　处：《实验动物与比较医学》2008年第2期99-101,共3页Laboratory Animal and Comparative Medicine

摘　　要：目的观察SHIV-B'whu感染中国猕猴的组织病理学变化。方法SHIV-B'whu感染中国猕猴,4次传代(P1￣P4),定时测量动物体温变化,对各代感染的猴在第14天处死,进行病理学解剖观察,并且对全身主要脏器以及多处淋巴结组织取材做病理分析。结果中国猕猴在感染急性期体温有短暂升高,经病理分析,4代感染猴均有典型的间质性肺炎特征,肝组织有点状或灶性坏死,淋巴窦扩张,淋巴滤泡萎缩,淋巴细胞减少,淋巴结出现噬红细胞现象,组织巨噬细胞免疫组化染色阳性率从P1到P4逐步增加,从3.5%到22%。结论SHIV-B'whu能成功感染中国猕猴,引起与HIV感染人后所相似的免疫病理学变化。Objective To study pathological changes on Chinese rhesus monkeys with recombined Simian/Human Immunodeficiency virus. Methods Chinese rhesus monkeys were inoculated with a serial of four in vivo passages （P1-P4） of SHIV-B＇WHU. Each serial were sacrificed on fourteenth day. Pathological changes of lymph nodes were studied respectively. Results The infected monkeys demonstrated a transient increase of body temperature during the acute stage of infection with all the passages of SHIM SHIV-B＇whu infection was also demonstrated by the pathological findings： focal necrosis in liver, interstitial pneumonia, partially enlarged lymph antrum, diminished lymph follicle, and erythrophagia in lymph nodes. Macrophage positive rates in monkey tissues increased at a uncertain gradient from P1 to P4（from about 3.5% to 22%）. Conclusion This study provide direct evidence that SHIV-B＇whu has the ability to infect Chinese rhesus monkeys, which can present the similar symptom as human being.

关 键 词：SHIV 感染 免疫 病理学变化 

分 类 号：R361.2[医药卫生—病理学]