两种大鼠急性坏死性胰腺炎模型制作方法的改良和比较  

Modification and Comparison of Two Methods for Creating Rats Models of Acute Necrotizing Pancreatitis

作　　者：刘纳新[1] 管敏强[2] 陈通克[2] 赵惠玲[2] 陈锡文[2] 

机构地区：[1]温州医学院附属第一医院,温州325000 [2]温州医学院动物实验中心,温州325600

出　　处：《实验动物与比较医学》2008年第2期110-113,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的比较两种大鼠急性胰腺炎动物模型制作方法的各自特点及其应用。方法40只SD大鼠随机分成胆胰管直接穿刺组(A组,n=16)、经十二指肠肠壁穿刺组(B组,n=16)和对照组(C组,n=8)。取A组、B组其中一半大鼠(分别为A1组和B1组)与C组一起在制模后24 h处死,观察各组手术时间、腹水量、淀粉酶含量和胰腺炎的严重程度,同时对A组、B组的剩余大鼠(分别为A2组和B2组)作4 d的生存分析。结果两种方法24 h胰腺病理改变均表现为急性坏死性胰腺炎;A1组和B1组的24 h淀粉酶和腹水量显著高于C组(P<0.01),而这些指标在A1组和B1组之间没有差异;A1组和B1组的制模时间存在差异(P<0.05);4 d内生存分析提示B2组生存时间明显长于A2组(P<0.05)。结论经十二指肠肠壁穿刺复制急性坏死性胰腺炎的方法明显优于胆胰管直接穿刺法;而胆胰管直接穿刺法由于操作简便,在某些短期研究仍可选用。Objective Compare two methods for creating the rat model of acute necrotizing pancreatitis and their application. Methods Fourty SD rats were randomly divided into biliopancreatic duct directly punctured group （A group, n =16）, duodenal wall punctured group （B group, n = 16） and control group （C group, n = 8）. One-half of the animals of A group and B group was sacrificed 24 h after operation （as A1 group and B1 group）, and the following parameters were examined： the model creating time, the volume of ascetic fluid, serum amylase and the severity of pancreatitis. Four days after operation, survival analysis of the remaining rats in A group and B group （as A2 group and B2 group） was performed. Results The histological manifestation of acute necrotizing pancreatitis was observed 24 h after operation in A1 group and B1 group; The serum amylase and the ascetic volume of A1 group and B1 were higher obviously than C group （P〈0.01）, and these parameters were no different between A1 group and B1 group; The model creating time between A1 group and B1 group were different （P〈0.05）, and the survival rate was significantly higher in B2 group than that in A2 group（P〈0.05）. Conclusion The method of puncturing duodenal wall is superior to that of directly puncturing biliopancreatic duct for creating acute necrotizing pancreatitis; while the later can be used in some short term studies because of its simple manipulation.

关 键 词：急性坏死性胰腺炎 大鼠模型 胆胰管 十二指肠壁 

分 类 号：R-332[医药卫生]