车前子多糖的测定方法研究  

Study on determination of the contents of polysaccharides in Semen Plantaginis

作　　者：周超[1] 谢明勇[1] 万茵[1] 聂少平[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《分析试验室》2008年第4期10-13,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(30660226);教育部长江学者和创新团队发展计划(IRT0540)项目资助

摘　　要：建立了一种准确测定车前子中多糖的方法。采用苯酚-硫酸法测定车前子中多糖量,以木糖作为标准对照物,检测波长480 nm。实验结果表明,车前子中多糖平均值为9.04%,平均加标回收率为98.2%,RSD=1.7%(n=6)。此测定方法适用于车前子多糖含量的测定,也可为其他戊糖含量高的多糖测定提供参考。A better method for the determination of the contents of polysaccharides in Semen Plantaginis was eatablished.The contents of polysaccharides in Semen Plantaginis were determined by Phenol-H2SO4 colorimetry at 480 nm with xylose as a standard.The results showed that the average value of content of polysaccharides in Semen Plantaginis was 9.04%,the mean recovery for the determination of the polysaccharides was 98.2% with the RSD of 1.71%（n=6）.This method was simple,accurate and reproducible.It is suitable for the determination of polysaccharides in Semen Plantaginis,also it provides reference for the determination of polysaccharides with high pentoses content from other plants.

关 键 词：车前子多糖 含量测定 苯酚-硫酸法 

分 类 号：O657.3[理学—分析化学]