高强度次声作用小鼠后海马内P53mRNA表达的变化  

P53 mRNA expression in hippocampus of mice exposed to high intensity infrasound

作　　者：牟翔[1] 袁华[1] 李玲[2] 刘静[1] 葛雪松[1] 瞿丽莉[1] 陈景藻[1] 

机构地区：[1]第四军医大学西京医院物理医学与康复科,西安710032 [2]中国人民解放军总院第一附属医院康复理疗科

出　　处：《中国康复医学杂志》2008年第4期320-321,共2页Chinese Journal of Rehabilitation Medicine

基　　金：全军医学科学技术研究“十五”计划指令性课题(OlL071)

摘　　要：目的:研究高强度次声作用小鼠后海马内P53mRNA表达的变化。方法:BALB/C小鼠暴露于16Hz声压130dB次声。每天作用2h,分别作用1、7、14、21和28d后,采用原位杂交的方法观察小鼠海马内P53mRNA表达的改变。结果:130dB次声作用后,海马区域内P53mRNA表达明显增多;在相同声压级强度的次声作用下,随着作用次数的增加,海马内P53mRNA的杂交阳性反应产物增多。结论:一定参数次声作用后,脑内P53mRNA表达的增高,提示脑组织的结构和功能发生变化,导致DNA损伤,神经元受到损害。这一效应与次声的声强和暴露时间有关。P53mRNA在次声导致脑组织损害过程中亦起着非常重要的作用。Objective：To explore the change of P53mRNA in the hippocampus of mice exposed to infrasound of high sound pressure.Method：BALB/C mice were exposed to infrasound of 16Hz 130dB for 2h everyday and lasted for 1, 7, 14, 21 and 28d respectively. In situ hybridization was used to study the expression of P53mRNA in hippocampus, Result： P53mRNA in hippocampus increased after exposed to 130 dB infrasound. Under the same sound pressure level, P53mRNA in hlppocampus increased along with the increase of exposure time,Conclusion： Hippocampus is sensitive to infrasound. By effects on P53 mRNA, infrasound may damage the brain. In the meantime, P53 mRNA is an important factor for the brain injury caused by infrasound, The effects related to the sound pressure level and exposure time of infrasound.

关 键 词：次声 脑损伤 P53MRNA 

分 类 号：R363[医药卫生—病理学]