Thrombospondin-1 expression correlates with angiogenesis in experimental cirrhosis  

作　　者：Gülsüm zlem Elpek Güzide Ayse Gkhan Sevgi Bozova 

机构地区：[1]Akdeniz University, Medical School, Department of Pathology, Antalya 07070, Turkey

出　　处：《World Journal of Gastroenterology》2008年第14期2213-2217,共5页世界胃肠病学杂志（英文版）

基　　金：Akdeniz University Scientific Research Foundation, 2004. 04. 0103. 013

摘　　要：AIM:To investigate the significance of Thrombospondin-1 (TSP-1) expression and its relationship with angiogenesis during experimental fibrosis. METHODS:Cirrhosis was induced in male Wistar rats by intraperitoneal administration of diethyl nitrosamine (DEN). The serial sections from liver tissues were stained with anti-CD34 and anti-TSP-1 antibodies before being quantitated by light microscopy. RESULTS:Our results showed that of TSP-1 expression gradually increases according to the severity of fibrosis (GroupⅠvs group Ⅱ, Group Ⅲ and Group Ⅳ;Group Ⅱ vs group Ⅲ and group Ⅳ;group Ⅲ vs group Ⅳ, P < 0.05). Moreover, TSP-1 expression was found to be correlated with angiogenesis (P < 0.05). CONCLUSION:The correlative evidence of the link between TSP-1 and fibrosis or angiogenesis provided by this study suggests that besides its role as a strong promoter of transforming growth factor-β1 (TGF-β1), TSP-1 might have an additional role in liver fibrogenesis by stimulating angiogenesis and this protein could be a potential target to prevent fibrogenesis in chronic inflammatory diseases of the liver.AIM：To investigate the significance of Thrombospondin-1 （TSP-1） expression and its relationship with angiogenesis during experimental fibrosis. METHODS：Cirrhosis was induced in male Wistar rats by intraperitoneal administration of diethyl nitrosamine （DEN）. The serial sections from liver tissues were stained with anti-CD34 and anti-TSP-1 antibodies before being quantitated by light microscopy. RESULTS：Our results showed that of TSP-1 expression gradually increases according to the severity of fibrosis （GroupⅠvs group Ⅱ, Group Ⅲ and Group Ⅳ;Group Ⅱ vs group Ⅲ and group Ⅳ;group Ⅲ vs group Ⅳ, P 〈 0.05）. Moreover, TSP-1 expression was found to be correlated with angiogenesis （P 〈 0.05）. CONCLUSION：The correlative evidence of the link between TSP-1 and fibrosis or angiogenesis provided by this study suggests that besides its role as a strong promoter of transforming growth factor-β1 （TGF-β1）, TSP-1 might have an additional role in liver fibrogenesis by stimulating angiogenesis and this protein could be a potential target to prevent fibrogenesis in chronic inflammatory diseases of the liver.

关 键 词：Experimental liver cirrhosis IMMUNO-HISTOCHEMISTRY Liver fibrosis Pathologic angiogenesis Thrombospondin-1 

分 类 号：R575.2[医药卫生—消化系统]