β-榄香烯脂质体体内外对消化系肿瘤的抑制作用  

In vivo and in vitro anti-tumor effects of β-elemene liposome

作　　者：王士勇[1] 杨振君[2] 于环[1] 杜微丽[1] 刘飒[1] 张哲[1] 张远[1] 邓英杰[3] 

机构地区：[1]中国医科大学附属第四医院生物治疗科,辽宁省沈阳市110032 [2]中国医科大学附属第二医院信息科,辽宁省沈阳市110004 [3]沈阳药科大学药学院,辽宁省沈阳市110016

出　　处：《世界华人消化杂志》2008年第10期1059-1063,共5页World Chinese Journal of Digestology

基　　金：教育部归国博士启动课题资助项目;No.2004-527~~

摘　　要：目的:评价β-榄香烯脂质体注射剂实验性治疗消化系肿瘤的作用.方法:用细胞培养方法测定β-榄香烯脂质体及其乳剂对5株人消化系癌细胞株和1株人胚肺成纤维细胞的半数抑制浓度(IC50);建立肝癌H22昆明鼠实体瘤和腹水模型,尾静脉注射高(80mg/kg)、中(40mg/kg)、低(20mg/kg)剂量β-榄香烯脂质体,测定其在动物体内的抑瘤率和生命延长率,注射葡萄糖和40mg/kgβ-榄香烯乳剂注射液作为阴性和阳性对照.结果:β-榄香烯脂质体及乳剂对5株人消化系癌细胞株的IC50分别为34.1±4.5mg/L-51.4±4.2mg/L和41.5±4.7mg/L-82.3±8.8mg/L,前者约为后者的1/2.在实体瘤鼠,高、中、低剂量β-榄香烯脂质体和乳剂的抑瘤率分别为47.4%-50.8%、38.2%-45.0%、23.2%-28.2%和21.8%-43.0%,平均瘤质量明显低于葡萄糖组(t>4.09,P<0.05).在腹水瘤鼠,脂质体和乳剂较葡萄糖组生存期延长51.3%-153.0%(t>7.92,P<0.05),中、高剂量脂质体组较乳剂组明显延长(t>4.64,P<0.05).β-榄香烯脂质体的刺激症状、尾静脉炎明显低于乳剂组.结论:β-榄香烯脂质体较乳剂有明显抗肿瘤作用和较低的副作用.AIM： To investigate the inhibitory effects of β-elemene liposome on digestive tumors in vitro and in vivo.METHODS： Five digestive tumor cell lines and one human lung embryonic fibroblast cell line were used to test the IC50 values of β-elemene liposome in vitro by cell culture. H22-bearing Kunming mice were treated with injection of β-elemene liposome through the tail vein at 20, 40 or 80 mg/kg to establish solid and ascites tumor models. Mice treated with glucose or elemene emulsion served as negative or positive controls, respectively. The anti-tumor effects, sur- vival and side effects of β-elemene liposome observed and compared between different groups. RESULTS： IC50 values of β-elemene liposome and elemene emulsion for the five digestive tumor cell lines were from 34.1 ± 4.5 to 51.4 ± 4.2 mg/L and 41.5 ± 4.7 mg/L to 82.3 ± 8.8 mg/L, respectively, and IC50 of the former was half of the later. In solid tumor models, the inhibitory rates of 80-, 40- or 20-mg/kg β-elemene liposome and elemene emulsion were 47.4%-50.8%, 38.2%45.0%, 23.2%-28.2% and 21.8%-43.0%, respectively, and the average tumor weights were lower than those in the glucose control group （t 〉 4.09, P 〈 0.05）. In ascites tumor models, the survival time was 51.3%-153.0% longer in β-elemene liposome or emulsion group than that in the glucose control group （t 〉 7.92, P 〈 0.05）. Furthermore, the survival time in 80- or 40-rag/ kg β-elemene liposome group was longer than that in elemene emulsion group （t 〉 4.64, P 〈 0.05）. The stimulating symptoms and phlebitis of tail vein generated by injection of β-elemene liposome were slighter than those by elemene emulsion. CONCLUSION： β-elemene liposome has greater ability to inhibit tumor growth and relieve side effects than elemene emulsion.

关 键 词：消化系统 肿瘤 Β-榄香烯 脂质体 抗肿 瘤作用 

分 类 号：R735[医药卫生—肿瘤]