灵芝孢子粉对人肝癌细胞HepG2及裸鼠移植瘤生长的抑制作用  

Inhibitory effect of Lucid Ganoderma spore on human hepatocarcinoma cell line HepG2 and growth of transplanted tumor in nude mice

作　　者：王顺官[1] 王筱婧[2] 李琳[2] 徐江平[2] 

机构地区：[1]广东省第二人民医院肿瘤放射治疗科,广东省广州市510317 [2]南方医科大学药学院药理学系,广东省广州市510515

出　　处：《世界华人消化杂志》2008年第10期1114-1118,共5页World Chinese Journal of Digestology

基　　金：广东省中医药管理局建设中医药强省课题资助项目;No.2006-31-1060014~~

摘　　要：目的:研究灵芝孢子粉对人肝癌细胞HepG2生长的抑制作用.方法:运用MTT法研究灵芝孢子粉水溶性成份在体外对HepG2细胞的抑制作用,并利用裸小鼠的移植瘤模型研究灵芝孢子粉在体内对HepG2细胞的生长抑制作用.HE染色观察肝癌组织结构和细胞形态改变.结果:TMTT实验结果表明灵芝孢子粉对HepG2细胞的IC50值随作用时间增加而减小,72h达到最低,为2.14g/L.体内抑瘤实验结果显示灵芝孢子粉为2000mg/kg时,对裸小鼠移植瘤的抑制率为57.0%.镜下观察灵芝孢子粉治疗组肿瘤坏死组织较多,细胞异型性较小.结论:高浓度及高剂量的灵芝孢子粉具有抑制肿瘤细胞生长的作用.AIM： To evaluate the inhibitory effects of Lucid Ganoderma spore （LGS） on human hepatocarcinoma cell line HepG2. METHODS： MTT assay was used to detect the in vitro inhibition of LGS on human hepato carcinoma cell line HepG2. Nude mouse model of human hepatocarcinoma was established and growth of the transplanted tumor was observed in vivo. The morphology of tumor cells was observed by microscopy after HE staining. RESULTS： MTT assay showed that the 50% inhibitory concentration （IC50） was decreased with the increasing of time. The minimum value of IC50 was observed at 72 h （2.14 g/L）. In vivo study revealed that LGS had an inhibitory action on HepG2 cells （dose： 2000 mg/kg, inhibitory rate： 57.0%）. The morphologic observation exhibited larger areas of necrosis and less degree of atypia afer LGS treatment. CONCLUSION： LGS has inhibitory action on human hepatocarcinoma cell line HepG2 at high concentrations and high dosages.

关 键 词：灵芝孢子粉 HEPG2 肝癌 四唑盐比色法 

分 类 号：R285[医药卫生—中药学] R735.7[医药卫生—中医学]