基质金属蛋白酶-9与抑癌基因PTEN在大肠腺瘤、大肠癌组织中的表达及意义  

Expression of matrix metalloproteinase-9 and tumor suppressor gene PTEN and their significances in colorectal carcinoma and adenoma

作　　者：袁禧先[1] 张丹[2] 王凤荣[1] 张琦玮[2] 

机构地区：[1]佳木斯大学附属第一医院消化二科,黑龙江省佳木斯市154002 [2]佳木斯大学临床医学院,黑龙江省佳木斯市154002

出　　处：《世界华人消化杂志》2008年第10期1132-1136,共5页World Chinese Journal of Digestology

基　　金：2007年研究生创新科研资助项目;No.YJSCX2007-0076HLJ~~

摘　　要：目的:探讨MMP-9、PTEN在大肠癌、大肠腺瘤、大肠正常组织中的表达,进一步了解二者的关系.方法:通过免疫组化EliVision方法检测基质金属蛋白酶-9(MMP-9)与PTEN在肠癌组、腺瘤组及正常黏膜组中的表达,分析MMP-9与PTEN在大肠癌发病中的相关性.结果:正常组MMP-9的表达率明显低于肠癌组和腺瘤组(15.00%vs56.50%,45.00%,均P<0.01),正常组PTEN的表达率明显高于肠癌组和腺瘤组(95.00%vs39.10%,65.00%,均P<0.01),二者在腺瘤组与肠癌组间比较差别均无显著性.MMP-9在肠癌组中表达与肿瘤的分化程度呈负相关,与浸润深度、淋巴结转移呈正相关,PTEN蛋白表达与此相反,二者均与肿瘤的大小、患者的年龄均无相关性.MMP-9与PTEN在大肠癌中的表达具有相关性(r=-0.797,P<0.05).结论:MMP-9与PTEN在大肠癌发生发展中起重要作用,二者可作为评估大肠癌浸润转移的重要生物学指标.AIM： To investigate the expression ot matrix metalloproteinase-9 （MMP-9）, PTEN in colorectal carcinoma, adenoma and normal mucosa of large intestine as well as their correlations. METHODS： EliVision immunohistochemical method was used to examine the expression of MMP-9 and PTEN in colorectal carcinoma, ade- noma and normal mucosa, and their correlations were also analyzed. RESULTS： The expression of MMP-9 was significantly lower in normal mucosa than that in carcinoma and adenoma （15.00% vs 56.50%, 45.00%;both P 〈 0.01）, while the expression of PTEN was significantly higher （95.00% vs 39.10%, 65.00%; both P 〈 0.01）. MMP-9 and PTEN expression were not significantly different between adenoma and carcinoma. MMP-9 expression was positively correlated with histological classification, but negatively correlated with lymph node metastasis and the depth of invasion. PTEN expression was just the opposite. Both MMP-9 and PTEN expression had no correlations with the age of patients and tumor size. There existed a significant correlation between the expressions of MMP-9 and PTEN in colorectal carcinoma （r =-0.797, P 〈 0.05）. CONCLUSION： The expression of MMP-9 and PTEN may play important roles in the occurrence and development of colorectal carcinoma. MMP-9 and PTEN may serve as new markers in the diagnosis and prognosis of colorectal carcinoma.

关 键 词：基质金属蛋白酶-9 PTEN 大肠腺瘤 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]