构建海人酸急性致痫小鼠模型  

Establishing the mouse models of acute epilepsy induced by kainic acid

作　　者：杨小岗[1] 刘卫平[1] 章翔[1] 王剑博[1] 姬西团[1] 

机构地区：[1]第四军医大学西京医院神经外科,西安在读硕士710032

出　　处：《立体定向和功能性神经外科杂志》2008年第2期90-93,共4页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：国家自然科学基金项目(No:30571911);第四军医大学西京医院高新项目(N0:XJGX03033M25)

摘　　要：目的建立海人酸诱导的小鼠急性癫痫模型,并探讨其特点。方法实验取健康雄性昆明小鼠99只,随机分为生理盐水组(n=33)和致痫组(n=66),痫组腹腔注射海人酸10mg/kg,生理盐水组腹腔注射生理盐水35μl/g。注射后连续5h观察小鼠是否有痫性发作并分级。当小鼠持续痫性发作达1h时给予地西泮4mg/kg腹腔注射,对照组3只和致痫组9只同时描记脑电图,并取脑切片后苏木精-伊红染色法观察海马各区病理学改变。结果①行为学表现,模型组小鼠注射海人酸后可出现湿狗样抖动、头面部肌肉阵挛、肢体阵挛及全面强直阵挛发作。生理盐水对照组未见癫痫发作。②脑电图表现,癫痫持续状态小鼠表现为持续性节律性棘波、棘慢波或高波幅慢波。③病理学研究,双侧海马均可出现神经元变性,以CA1和门区为主。结论腹腔注射海人酸致痫小鼠急性模型同相应的大鼠模型一样,具有制作简便、痫性发作潜伏期短、致痫率高等特点,其所产生的急性癫痫模型具有与人类颞叶癫痫相似的行为、脑电图与神经病理改变。Objective To establish the mouse models with acute epilepsy induced by kainic acid,and explore the characteristics. Methods 99 healthy male Kunming mice were selected and divided randomly into the saline group （n=33） and seizure-induced group（n=66）. The seizure -induced group were treated with 10mg/kg kainic acid by intraperitoneal injection,and those in the saline group were treated with 35μl/g saline by intraperitoneal injection. After injection, the following 5 hours if there was seizure or not was observed continuously and was graded. When the seizure lasted for I hour,the mice were offered 4mg/kg diazepam by intraperitoneal injection. 3 in the control group and 9 in the seizure-induced group were monitored by electro encephalogram （EEG）. The mice brains were sectioned and the pathologic change in the hippocampal area was checked by hemotoxylin-eosin（HE） staining. Results Totally 99 mice entered the final analysis. （1）On the praxiology, mice in model group showed wet dog shakes, the clonus of face, head and limbs,and generalized tonic-clonic convusions after injection of kainie acid. Epileptic seizure was not observed on mice in saline control group. （2）On the EEG, the mice of the status epilepticus could see the explosive slow wave of high amplitude of wave, spike wave or spike slow wave. （3）On the pathology, intraperitoneal injection could lead to neuronal degeneration in the bilateral hippocampus,mainly in the CA1 and hilar area. Conclusion The kaini cacid induced mouse model swith acute epilepsy is the same tothe corresponding mice models with the characters of easy to make,short latency of seizure and high ineidenee mice etc. The developed acute epilepsy models have mimic features of human temparal lobe epilepsy, and the brain electric wave and neural pathology have changed.

关 键 词：海人酸 癫痫 小鼠 疾病模型 

分 类 号：R742.1[医药卫生—神经病学与精神病学]