长期饮酒减少大鼠睾丸间质细胞PBR和StAR蛋白表达  

Decreased protein expressions of PBR and StAR of Leydig cells in rats with chronic ethanol feeding

作　　者：汪海东[1] 郑冬梅[1] 冯丽[1] 侯晓磊[1] 高聆[1] 赵家军[1] 

机构地区：[1]山东大学临床医学院山东省立医院内分泌科,济南250021

出　　处：《中华内分泌代谢杂志》2008年第2期200-203,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：山东省自然科学基金资助项目（Y2001C12）

摘　　要：目的研究慢性饮酒对雄性大鼠睾丸外周型苯二氮[艹卓]类受体（PBR）和类固醇生成快速调节蛋白（StAR）表达的影响。方法以不同浓度的乙醇饲养40只Wistar大鼠20周，检测睾丸组织PBR和StAR蛋白的表达。结果慢性饮酒Wistar大鼠的睾丸曲精小管生精细胞层明显减少，管腔中可见断裂的精子鞭毛，少见完整的精子；免疫沉淀显示PBR和StAR蛋白表达下降，与对照组相比较，小、中、大剂量饮酒组PBR和StAR表达分别下降13．8％、20．9％、50．4％和34．5％、37．7％、95．2％；免疫组化显示小、中、大剂量饮酒组睾丸间质组织中的PBR和StAR表达面积分别减少33．27％、37．71％、63．59％和27．12％、51．84％、58．41％。结论慢性饮酒能降低睾丸间质PBR和StAR蛋白表达，且其与乙醇浓度呈正相关。Objective To investigate the expressions of peripheral type benzodiazepine receptor （PBR） and steroidogenic acute regulatory protein （STAR） of testis in rats with chronic ethanol feeding. Methods Forty rats were treated with different ethanol dosages for twenty weeks, the morphology of testis and protein expressions of PBR and StAR were observed. Results In the ethanol-feeding rats, seminiferous tubular wall of testes became thin and the layer of germ cells was significantly reduced, moreover, the broken spermatozoon＇s flagella were frequently observed and few integrated spermatozoa were produced. Compared with control group, the protein expressions of PBR and StAR protein were reduced by 13.8%, 20.9%, 50.4% and 34.5%, 37.7%, 95.2% in low-, middle- and high-dose ethanol feeding group respectively by immunoprecipitation. Similarly, both locating at interstitial cells in testes were also decreased by 33.27 %, 37.71%, 63.59 % and 27.12 %, 51.84 %, 58.41% in the same ethanol feeding groups respectively by immunohistochemistry. Conclusion Both PBR and StAR protein expressions are decreased in interstitial cells of testes in chronic ethanol-feeding rats, which shows positive correlation with ethanol dosage.

关 键 词：乙醇 受体 苯二氮[艹卓] 类固醇生成快速调节蛋白 大鼠 WISTAR 

分 类 号：R363[医药卫生—病理学]