胎盘免疫调节肽遗传毒性和抗突变作用的研究  

Study on genotoxicity and antimutagenic effects of the placental immunoregulation polypeptide

作　　者：丰慧根[1] 李延兰[1] 杨保胜[1] 刘明林[1] 任太芳[2] 潘莹[2] 

机构地区：[1]新乡医学院细胞生物学教研室,河南新乡453003 [2]新乡医学院一附院

出　　处：《新乡医学院学报》1997年第3期266-268,271,共4页Journal of Xinxiang Medical University

基　　金：河南省教委资助

摘　　要：目的研究胎盘免疫调节肽（PIP）的遗传毒性和抗突变作用。方法应用体外培养人淋巴细胞并进行微核、染色体畸变检测以及小鼠骨髓微核实验的研究，评价PIP的遗传毒性和抗突变的效应。结果PIP（18μg·ml-1和27μg·ml－1）可显著抑制培养人淋巴细胞的自发和γ─射线诱发的微核形成（P＜0.01）以及丝裂酶素C（MMC）诱发的染色体畸变（P＜0．01），并能明显抑制环磷酰胺诱发的小鼠骨髓多染性红细胞微核（PEC-MN）的增加（P＞0．01）。结论PIP无遗传毒性，具有抗突变作用。To investigate the genotoxicity and Antimutagenic effects of placetntal immunoreguLation ploypeptide.(PIP) Method The genotoxicity and antimutagenic Effects of PIP at various concentration was deterMined in human lymphocytes micronucleus formAtion test,chromosomol aberrations test and bone Marrow micronucleus test in mice.Results PIP(18μg and 27μg·ml-1)signifiCantly inhibited spontaneous and r-rays-induced Micronucleus formation and MMC-induced chroMosomal aberrations in human lymphocyties in vitro.Meantime PIP(1.8mg and 27mg)suppressed strongly micronucleus formation induced by CP in PCEs of mice. Conclusion PIP haven't genotoxicity and has Antimutagenic effects.

关 键 词：胎盘免疫调节肽 抗突变 遗传毒性 染色体畸变 微核 

分 类 号：R392.2[医药卫生—免疫学]