Treg细胞对细胞毒性T淋巴细胞抗胃癌效应中释放穿孔素、干扰素的影响  

Treg suppressing CTL anti-gastric cancer effect by restraining perforin and IFN-γ releasing

作　　者：曾冬竹[1] 雷晓[1] 石彦[1] 郑峻松[2] 罗华星[1] 余佩武[1] 

机构地区：[1]第三军医大学西南医院普通外科肿瘤生物治疗中心,重庆400038 [2]第三军医大学医学检验系临床检验学教研室

出　　处：《中华实验外科杂志》2008年第4期420-421,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30200264）

摘　　要：目的观察Treg细胞对细胞毒性T淋巴细胞（CTL）抗胃癌效应中释放穿孔素、干扰素的影响。方法构建CTL细胞对MFC胃癌细胞有效杀瘤体系，加入0．5×10^5到50．0×10^5不同剂量的Treg细胞，观察CTL杀瘤活性抑制并检测穿孔素、干扰素浓度。结果Treg数量达到5．0×10^5后CTL杀瘤活性受明显抑制，穿孔素、干扰素浓度明显降低（P〈0．05）。结论Treg可通过降低CTL细胞分泌穿孔素、干扰素而抑制其杀瘤活性。Objective To investigate the in vitro inhibitory effect of Treg on restraining the CTL＇s anti-gastric cancer perforin and IFN-γ releasing. Methods MFC tumor killing system was established in vitro with CTL. Treg in different dosages （0.5 × 10^5 to 50.0 × 10^5 ） was added. The inhibitory effect was studied by monitoring the changes of perforin and IFN-γ. Results The killing effect was obviously suppressed when Treg reached the amount of 5.0 × 10^5. Levels of perforin and IFN-γ were significantly decreased （P 〈 0.05）. Conclusion Treg can suppress antitumor effect of CTL by restraining perforin and IFN-γ releasing.

关 键 词：胃癌 穿孔素 干扰素 

分 类 号：R735.2[医药卫生—肿瘤]