PRL-3 miRNA重组慢病毒对胃癌SGC7901细胞增殖的抑制作用  

Transfection of PRL-3 miRNA lentivirus suppressed proliferation of human SGC7901 gastric cancer cell line

作　　者：蔡世荣[1] 陈创奇[1] 王昭[1] 崔冀[1] 张常华[1] 何裕隆[1] 詹文华[1] 

机构地区：[1]中山大学附属第一医院胃肠胰外科/中山大学胃癌诊治中心,广州510080

出　　处：《中华实验外科杂志》2008年第4期431-433,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30571833）;中国博士后科学基金资助项目（2005038448）

摘　　要：目的观察PRL-3在人胃癌SGC7901细胞增殖中的作用。方法构建表达人工PRL-3 miRNA的慢病毒载体，转染SGC7901细胞，并检测其沉默PRL-3表达的效果；MTT试验评价沉默PRL-3表达对SGC7901细胞增殖的抑制作用。结果成功构建表达PRL-3慢病毒载体。PRL-3慢病毒转染组，可沉默SGC7901细胞PRL-3的表达。MTT试验结果，沉默PRL-3的表达后，与空载体组比较，SGC7901细胞数下降了30．4％；生长曲线比较，SGC7901细胞的增殖受到明显抑制（P〈0．05）。结论PRL-3在胃癌生长中起着重要作用，可作为胃癌潜在的治疗靶点。Objective To determine the role of PRL-3 in the proliferation of human SGC7901 gastric cancer cell line. Methods RNA interference,mediated by recombinant lentivirus expressing artificial PRL-3 miRNA, was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay in vitro was conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells. Results Recombinant lentivirus expressing artificial PRL-3 miRNA （ Lenti. rPRL3-miRs） was construtted successfully. Transduction of PRL-3 miRNA lentivirus effectively silenced the PRL-3 expression in SGC7901 cells. Knockdown of PRL-3 significantly suppressed the proliferation of SGC7901 cells in vitro, and the transfeeted cells were decreased by 30.4% as compared with vacant vector group （ P 〈 0.01 ）. Conclusion PRL-3 plays a key role in the growth of gastric cancer cell,and PRL-3 silencing inhibits gastric cancer cell growth. PRL-3 can be used as a potential therapeutic target to treat gastric cancer.

关 键 词：胃肿瘤 RNA干扰 慢病毒 

分 类 号：R735[医药卫生—肿瘤]