Cdx2对胃癌细胞生物学性状的影响  

The effects of Cdx2 on biological characters of human gastric carcinoma cell line

作　　者：马玉林[1] 肖强[1] 谢玉波[2] 李雷[1] 唐振勇[1] 尹永硕[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,南宁530021 [2]广西医科大学第一附属医院麻醉科,南宁530021

出　　处：《中华实验外科杂志》2008年第4期437-439,共3页Chinese Journal of Experimental Surgery

基　　金：广西科学基金资助项目（桂科回0448013）

摘　　要：目的观察Cdx2对人胃癌细胞MGC-803生物学性状的影响。方法利用脂质体将pCMV—Cdx2-HA和pCMV-HA质粒分别转染MGC-803细胞，应用逆转录-聚合酶链反应（RT—PCR）和Western blot技术检测MGC-803细胞中Cdx2基因的表达；应用体外实验及流式细胞仪分别检测Cdx2对MGC-803的侵袭力、黏附力、增殖力、迁移力和凋亡率的影响。结果转染pCMV—Cdx2-HA质粒的MGC-803细胞中可以检测到高Cdx2的表达，其侵袭能力[穿膜细胞数：（64．33±11．94）个／视野下降到（23．93±8．95）个／视野]、黏附能力[（1．172±0．042）]、增殖力[（26．13±1．60）％]和迁移能力[（42．87±2．19）％]较对照组明显降低（P〈0．05），而且凋亡率升高，48h和72h的凋亡率分别为（11．40±0．36）％、（9．72±0．50）％（P〈0．05）。结论Cdx2高表达对MGC-803具有抑制其侵袭转移的作用，并促进肿瘤细胞的凋亡。Objective To explore the effects of Cdx2 on biological characters of human gastric carcinoma cell line MGC-803. Methods A eukaryotic expression vector was constructed to transfect a human gastric carcinoma cell line MGC-803. In MGC-803 cells transfected by pCMV-Cdx2-HA, Cdx2 gene was detected by RT-PCR and Western blot techniques. Cell matrigel invasion assay, adhesion assay, scratching assay, cloning assay, MTT method and apoptosis rate were used to study the effects of Cdx2 gene. Resuits Cdx2 gene was detected in MGC-803 cells transfected by pCMV-Cdx2-HA. All assays showed that the invasiveness （64.33 ± 11.94/vision to 23.93 ± 8.95/vision）, adhesion （1. 172 ±0. 042） ,proliferation （26.13 ± 1.60） % and migration （42.87 ± 2.19） % of MGC-803 cells transfected by pCMV-Cdx2- HA were less than those of MGC-803 cells transfected by pCMV-HA and untransfected cells （P 〈 0.05）. Apoptotic rate of MGC-803 cells transfected by pCMV-Cdx2-HA was higher than that of MGC-803 cells transfected by pCMV-HA and untransfected cells （ P 〈 0.05 ）, and apoptotic rate was （11.40 ± 0.36）% at 48 h and （9.72 ± 0.50） % at 72 h respectively. Conclusion Cdx2 gene can inhibit the invasion, migration, adhesion and proliferation of MGC-803 cells, and can promote apoptosis in vitro.

关 键 词：胃癌 转染 生物素 

分 类 号：R735.2[医药卫生—肿瘤] R574.62[医药卫生—临床医学]