丁酸钠对人肝癌细胞株SMMC-7721的诱导分化作用及其机制  

Effect of sodium butyrate on the apoptosis induction of human hepatoma cell line 7721

作　　者：王波涌[1] 钱群[1] 雷道雄[1] 艾中立[1] 张中林[1] 黄梅[2] 

机构地区：[1]武汉大学中南医院普外科,430070 [2]华中科技大学同济医学院附属同济医院血液科

出　　处：《中华实验外科杂志》2008年第4期458-460,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨丁酸钠（Sodium Butyrate，NaB）抑制人肝癌细胞株SMMC-7721细胞生长、诱导其凋亡的分子机制。方法采用噻唑蓝（MTT）比色法、倒置显微镜观察药物对细胞生长的影响并绘制细胞增殖抑制曲线；透射电镜观察细胞凋亡的形态变化；流式细胞术分析细胞周期；半定量RT-PCR方法检测细胞p21WAF1基因mRNA的表达水平；Western blot检测细胞p21WAF1蛋白的表达变化。结果NaB对人肝癌细胞生长的抑制呈剂量依赖和时间依赖关系。将细胞周期阻滞于G0／G1期，NaB上调p21WAF1 mRNA及蛋白的表达。结论NaB具有抑制人肝癌SMMC-7721细胞增殖、诱导其凋亡的作用，这种作用可能是通过上调p21WAF1 mRNA及蛋白完成的。Objective To explore the molecular mechanisms of sodium butyrate （NAB） inducing apoptosis of human hepatoma cell line 7721. Methods The hepatoma cells were treated with various concentrations of NaB in different durations in vitro. Proliferation suppression was observed by MTT method and invert microscope, and morphological changes of apoptosis were observed by transmission electron microscopy （TEM）. Flow cytometry （FCM） was used to investigate the apoptoais rate and the cell cycle. The expression of p21 mRNA and protein was detected by semi-quantitative RT-PCR and Western-blot respectively. Results The NaB inhibited the growth of hepatoma cells in a dose-dependent and time-dependent manner. Invert microscopy and TEM showed that the cells treated with NaB exhibited characteristics of apoptosis. NaB blocked cells mainly in the G0/G1 phase, but stimulated p21 expression both at the mRNA and protein levels. Conclusion NaB effect on proliferation inhibition and apoptosis induction may be linked to its ability to up-regulate of p21 gene.

关 键 词：癌 肝细胞 丁酸钠 脱噬作用 分化 

分 类 号：R735.7[医药卫生—肿瘤] R733.7[医药卫生—临床医学]