桑皮苷A对照品的制备研究  

Preperation of reference substance of mulberroside A from root bark of Morus alba

作　　者：舒树苗[1] 潘勤[2] 肖峰[2] 朱小兰[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中新药业研究中心,天津300457

出　　处：《中草药》2008年第4期535-536,共2页Chinese Traditional and Herbal Drugs

摘　　要：目的研究从桑白皮中制备桑皮苷A对照品的制备方法。方法结合大孔树脂吸附、硅胶柱色谱和凝胶柱色谱对桑白皮提取物进行分离、纯化,利用TLC和HPLC-ELSD对分离产物进行质量分数检测。并通过各种波谱手段等对其进行结构确证。结果从桑白皮中分离、纯化出桑皮苷A对照品,质量分数>98.5%。结论该方法制备出的桑皮苷A对照品符合中药化学对照品的相关要求.可作为桑白皮药材和含桑白皮成药质量控制.以及中药药效物质基础研究用的化学对照品。Objective To establish a separation method for reference substance of mulberroside A from the root bark of Morus alba. Methods Ethanol-extract of the root bark of M. alba was isolated and purified by macroporous resin, silica gel, and Sephdex LH-20 column chromatography. The purity of mulberroside A was identified by TLC and ELSD-HPLC. Its structure was identified by spectral analysis. Results The compound was completely separated from the root bark of M. alba. The purity of the reference substance was not less than 98. 5%. Conclusion The compound prepared by the above-mentioned method is accorded with the relative demands of chemical reference substance in Chinese materia medica. It can be used as a reference substance for the quality control and the research of herbal medicine.

关 键 词：桑白皮 桑皮苷A 对照品 

分 类 号：R284.2[医药卫生—中药学]