溶菌酶与顺铂联合应用对肝癌SMMC-7721细胞的协同抑制效应  

Synergetic inhibition effects of lysozyme and cisplatin against proliferation of human liver cancer SMMC-7721 cells

作　　者：王利娟[1] 李琳娜[1] 何金花[1] 李海城[1] 刘誉[1] 

机构地区：[1]暨南大学医学院生物化学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2008年第2期130-133,142,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：暨南大学科研基金资助项目(51204004)

摘　　要：目的:探讨溶菌酶(lysozyme,Ly)和顺铂(cisplatin,Cis)对肝癌细胞SMMC-7721生长的协同抑制效应及其抑制机制,并分析Ly对正常肝细胞LO2的毒性作用。方法:分别用MTT比色法和RT-PCR观察Ly与Cis联合应用对SMMC-7721细胞生长的抑制作用以及对PCNA、bcl-2的mRNA表达水平的影响,同时测定Ly对LO2细胞生长的毒性作用。流式细胞仪观察Ly与Cis联合应用对SMMC-7721细胞诱导凋亡的作用。结果:Ly能有效地协同Cis抑制SMMC-7721细胞生长,抑制率最低36%,最高82%,呈时间、剂量依赖性关系,但在同样浓度下对LO2细胞几乎没有抑制作用。联合用药组的PCNA、bcl-2的mRNA的表达水平均比对照组明显降低。流式细胞仪分析显示,Ly协同Cis作用可诱导SMMC-7721细胞凋亡,最高凋亡率为32.5%。结论:溶菌酶(Ly)能有效地协同Cis抑制SMMC-7721细胞增生并促进其凋亡,其机制可能与Ly协同Cis降低PCNA和bcl-2的mRNA表达有关。Ahn：To study the effect of lysozyme combined with conventional cytotoxic agent cisplatin on proliferation of human liver cancer cells SMMC-7721, and to evaluate the toxicity of lysozyme in normal human liver cell line LO2. Methods： MTT assay was used to determine the inhibitory effect of the drugs on SMMC-7721 cells and RT-PCH used to determine the mRNA levels of PCNA and hcl-2. The toxic effect of lysozyme on proliferation of LO2 cells was also evaluated by MTT assay, and apoptosis of the cells incubated with or without drugs was analyzed by flow cytometry. Results： Our results showed that a significantly synergetic nhibitory effect on proliferation of SMMC-7721 cells was produced by combination of lysozyme with cisplatin, in a time-and dose-dependent manner. The inhibition rate ranged from 36% to 82%. The mRNA levels of either PCNA or hcl-2 in SMMC-7721 cells were found decreased in the group of lysozyme combined with cisplatin, and apoptosis of the cells was found to be evidently induced by the combination of these two agents in a dose-dependent manner,tne greatest apotosis rate is 32. 5%. Conclusion： Combination of lysozyme with conventional cytotoxic agent cisplatin can synergistically inhibit proliferation of SMMC-7721 cells in a time-and dos-dependent manner, and significantly induce apoptosisof the cancer cells. These may be resulted from the deceased expression of PCNA and bcl-2 in SMMC- 7721 cells caused by lysozyme-cisplatin combination.

关 键 词：鸡蛋清溶菌酶 顺铂 SMMC-7721细胞 凋亡 

分 类 号：R73-36[医药卫生—肿瘤]