选择性环氧合酶-2抑制剂对大鼠溃疡性结肠炎作用的实验研究  

Study of celecoxib on rat ulcerative colitis induced by trinitrobenzene sulfonic acid

作　　者：金磊[1] 曹勤[1] 陈锡美[1] 

机构地区：[1]上海市普陀区中心医院消化内科,200062

出　　处：《中国实用医药》2008年第12期49-50,共2页China Practical Medicine

摘　　要：目的明确选择性环氧合酶COX-2抑制剂塞来昔布对2、4、6三硝基苯磺酸(TNBS)诱导的大鼠溃疡性结肠炎的作用,并初步探讨其作用机制。方法将大鼠分为三组,第1组和第2组为造模组,用0.25 ml TNBS乙醇溶液(TNBS浓度25 mg/ml,乙醇浓度50%),灌肠,诱导大鼠实验性溃疡性结肠炎模型。造模组大鼠于造模前3 h开始,分别给予塞来昔布(12.5 mg/kg)和蒸馏水(1 ml/0.3 mg),2次/d,共7 d。第3组为正常对照组。第7 d实验结束时处死所用存活动物,观察结肠黏膜损伤程度,并用ELISA法检测IL-1β、IL-10、TNF-β的浓度。结果造模组结肠损伤积分为(8.20±1.96)(第1组)和(12.05±2.30)(第2组)均显著高于正常对照组(0.64±0.15)(P值均<0.01)。第1组的结肠损伤积分显著低于第2组(P<0.05)。第1组中IL-1β、TNF-β的表达分别为(5.27±1.13)pg/ml、(5.12±1.62)pg/ml较第2组的(6.76±2.01)pg/ml、(7.96±2.12)pg/ml低(P<0.05),但均高于正常对照组;而IL-10的表达第1组为(2.38±0.31)pg/ml较第2组(1.56±0.84)pg/ml高(P<0.05),均低于正常对照组。结论选择性COX-2抑制剂塞来昔布使大鼠实验性溃疡性结肠炎损伤减轻,对IL-1β、IL-10、TNF-β的表达有不同的影响。Objective An investigation was conducted to assess the effects and mechanism of celecoxib [ a selective cyclooxygenase （COX）-2 inhibitor] on a rat ulcerative colitis model induced by trinitrobenzene sulfonic acid （TNBS）. Methods The rats were divided into three groups . Group 1 and Group 2 were experimental groups. Ulcerative colitis was conducted by TNBS （25 mg/ml） in a vehicle of 50% ethanol （0.25 ml） in rats. Three hours before induction of ulcerative colitis, the rats were beginning and continuing to treat orally with celecoxib（12. 5 mg/kg）and distilled water （ 1 mL/0. 3 Mg） twice per day for 7 days. Group 3 was healthy control group. All rats that survived at the end of the experiment （7 d） were killed and the severity of damage were assessed. The IL-1β, IL-10,TNF-βconcentrations were tested by ELISA. Results The colonic damage scores were8.20 ± 1.96 in group 1 and （ 12.05 ±2. 30） in group 2. Both were significantly higher than that of group 3 （0. 64 ±0. 15） （P 〈0.01 ）. The colonic damage score of group 1 was significantly lower than that of group 2 （P 〈0.05）. The concentrations of IL-1β ,TNF-βin group 1 were, （5.27±1.13） pg/ml,（5.12±1.62） pg,/ml lower than（6. 76±2. 01 ）pg/rnl, （7. 96 ＋2. 12）pg,/rnl in group 2 and the concentrations of IL-10 in group 1 was（2. 38± 0. 31 ） pg,/ml higher than （ 1.56 ±0. 84） pg,/m in group 2 . Conclusion These results suggest that treatment with celecoxib （selective COX-2 inhibitor） may protect in experimental ulcerative colitis induced by TNBS.

关 键 词：溃疡性结肠炎 选择性环氧合酶-2抑制剂 炎症介质 

分 类 号：R574.62[医药卫生—消化系统]