牙骨质附着蛋白对猴骨髓基质细胞增殖和矿化能力的影响  

Effects of cementum attachment proteins on the proliferation and mineralization of monkey bone marrow stromal cells in vitro

作　　者：谢玉峰[1] 宋忠臣[1] 束蓉[1] 孙颖[1] 罗敏[2] 张秀丽[3] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙周科,200011 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所 [3]上海市口腔医学研究所,上海市口腔医学重点实验室

出　　处：《中华口腔医学研究杂志（电子版）》2008年第1期11-14,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：上海市科学技术委员会资助课题(054119525)

摘　　要：目的观察牙骨质附着蛋白(CAP)对体外培养的猴骨髓基质细胞增殖及矿化能力的影响。方法抽取猴髂骨骨髓,全血培养法获得骨髓基质细胞。培养液中CAP的浓度分别为0.125、0.25、0.5、1、2μg/ml,以不加CAP为空白对照。MTT法测定各组细胞的增殖活性,同时检测细胞内碱性磷酸酶活性。采用SAS6.12软件对实验数据行单因素方差分析。结果从第3天开始,0.5、1、2μg/mlCAP组与对照组相比能显著促进细胞增殖。对照组与不同浓度CAP实验组对细胞内碱性磷酸酶合成差异无统计学意义。结论0.5～2μg/ml的CAP都能显著促进体外培养的猴BMSCs增殖,但各浓度组CAP对细胞内碱性磷酸酶合成无影响。Objective The aim of this study is to investigate the effects of cementum attachment protein （CAP） on the proliferation and mineralization of cultured monkey bone marrow stromal cells （BMSCs） in vitro. Methods BMSCs were obtained from monkey bone marrow aspiration and cultured in DMEM medium with 10% FBS. The third passage cells were exposed to various concentrations of CAP （0.125, 0.25, 0.5, 1 and 2 μg/ml）. Controls were BMSCs cultured in DMEM medium without CAP. BMSCs proliferation rates and alkaline phosphatase （ALP） synthesis were assessed by an MTF assay. Statistical analyses were performed with a parametric one-way analysis of variance （ANOVA） and Stument-Newman-Keuls test for multiple comparisons. Results The proliferation rate of BMSCs was significantly up-regulated by CAP at concentrations of 0.5 to 2 μg/ml （P 〈 0.05） compared with the control group. There was no significant difference between the control group and experimental groups in ALP synthesis. Conclusion CAP could promote the proliferating ability of monkey BMSCs in vitro, whereas have little effects on ALP synthesis.

关 键 词：牙骨质附着蛋白 猴骨髓基质细胞 增殖 矿化 

分 类 号：R78[医药卫生—口腔医学]