三种铸造冠金属离子释放及其对细胞DNA损伤的实验研究  

Study of released metal ions of three kinds of dental casting alloy crowns and DNA damage of mucosal cells in vitro

作　　者：苏俭生[1] 田芝娟[1] 郭珊[1] 邵磊[2] 

机构地区：[1]同济大学口腔医学院,上海200072 [2]同济大学海洋地质国家重点实验室

出　　处：《中华口腔医学研究杂志（电子版）》2008年第1期26-29,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：上海市科委科技攻关重点资助项目(054119533)

摘　　要：目的探讨狗戴用纯钛、钴铬钼合金、镍铬合金三种铸造全冠后对其颊黏膜上皮细胞DNA损伤的情况。方法给狗分别进行纯钛、钴铬钼合金、镍铬合金三种铸造全冠修复,建立实验动物模型。采用IRIS Intrepid型电感耦合等离子体原子发射光谱仪检测戴冠前、戴冠2周、1个月、2个月及3个月时颊黏膜上皮细胞中金属离子的种类和浓度,同时应用彗星电泳方法检测颊黏膜上皮细胞DNA损伤情况。采用方差分析以及Spearman等级相关进行统计学分析。结果戴用纯钛全冠后,各实验阶段狗颊黏膜上皮细胞中金属离子浓度以及彗星数目变化差异无统计学意义(P>0.05);戴用钴铬钼合金全冠后,铬与钴离子浓度随着时间逐渐升高,其中戴冠2周、1个月及2个月各时间段间离子浓度差异有统计学意义(P<0.01),戴冠3个月与2个月之间差异无统计学意义(P>0.05),而钼离子浓度在戴冠前后差异无统计学意义(P>0.05);戴用镍铬合金全冠后,铬、镍及铍离子浓度随着时间逐渐升高,其中戴冠2周、1个月及2个月各时间段间离子浓度差异有统计学意义(P<0.01),戴冠3个月与2个月之间差异无统计学意义(P>0.05)。而钼与铝离子浓度在戴冠前后没有明显变化(P>0.05)。结论纯钛、钴铬钼合金全冠戴用后没有引起狗颊黏膜上皮细胞DNA的损伤,而戴用镍铬合金全冠后,狗颊黏膜上皮细胞DNA发生明显损伤。Objective To study the corrosion behavior of three kinds of dental casting alloys and investigate the DNA damage of dog bueeal mueosal cells caused by the released metal ions. Methods Three kinds of commonly-used dental casting alloys （pure Ti, Co-Cr-Mo and Ni-Cr） were used to make full crowns for dogs. The type and concentration of the released metal ions were measured by ICP-AES 2 weeks, 1 month, 2 months and 3 months after restoration. The DNA damage of buecal mucosal cells was studied by SCGE. Data were statistically analyzed by analysis of variance （ANOVA） and Spearman correlation. Results The metal ions released from Co-Cr-Mo and Ni-Cr were detected in bueeal mueosal cells while the amount of the ions released from the pure Ti was too small to be detected. The DNA damage of mucosal cells increased with time after restoration of Ni-Cr crowns. Conclusions Pure Ti was the most corrosion-resistant among the thee alloys. Pure Ti and Co-Cr-Mo showed no eytotoxicity. Ni-Cr alloy was prone to corrode and has cytotoxieity,

关 键 词：铸造合金 离子释放 细胞 DNA 

分 类 号：R783.3[医药卫生—口腔医学]