肿瘤细胞裂解物致敏的树突状细胞用于大鼠卵巢上皮癌免疫预防的体内研究  

The research of rat ovarian cancer in vivo immunotherapy using tumor cells lysates pulsed dendritic cells

作　　者：翟妍[1] 张震宇[1] 王淑珍[1] 乔宝丽[1] 

机构地区：[1]首都医科大学附属北京朝阳医院妇产科,100020

出　　处：《中国妇幼保健》2008年第13期1850-1852,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助项目(编号:39970770)

摘　　要：目的:通过负载肿瘤冻融抗原的树突状细胞(DC)预防大鼠卵巢上皮癌发生的体内试验,进行DC疫苗用于卵巢癌防治的可行性研究。方法:取大鼠骨髓单个核细胞(bone marrow mononuclear cells,BMMNC)体外培养获得成熟DC,诱导BMMNC细胞分化的第3天加入大鼠卵巢癌细胞株NuTu-19的冻融抗原,获得负载肿瘤抗原(TAA)的成熟DC;将致敏的DC制成DC疫苗经尾静脉注射大鼠体内,后于大鼠皮下接种NuTu-19细胞,观察大鼠皮下肿瘤的生长情况;注射生理盐水、NuTu-19冻融抗原、未致敏DC作为对照组。结果:大鼠骨髓来源的BMMNC在相关细胞因子的作用下可以培养出成熟的DC。TAA致敏的DC疫苗体内试验中肿瘤发生率较低,成瘤时间较晚,肿瘤生长速度缓慢,与对照组有显著性差别(P<0.01)。结论:大鼠骨髓来源的BMMNC可以培养出成熟的DC。DC可负载并提呈肿瘤抗原,体内试验证明负载癌抗原的DC可以杀伤肿瘤细胞,DC疫苗能够对肿瘤的发生起到主动免疫预防作用,在今后的肿瘤免疫防治方面有着广阔的前景。Objective： To study the feasibility of ovarian tumor cells lysates pulsed dendrite cells in the immunotherapy of tumor prevention in rats. Methods： The bone marrow mononuclear cells （BMMNC） were obtained to sample the mature DC by BMMNC cultured in medium with granulocyte - macrephage colony stimulating factor （ GM - CSF）, interleukin -4 （IL -4） and tumor necrosis factor - α. After culturing for 3 days, the rat＇s ovarian cancer cell line NuTu - 19 freeze - melt antigens were added to pulse DC and acquire the tumor cells lysates pulsed DC. Tumor cells NuTu - 19 were injected to the rats subcutaneous after vaccinating tumor cells lysates pulsed DC via tail vein, and the growth of subcutaneous tumor was observed. For control, saline, NuTu - 19 freeze - melt antigen and not - pulsed DC were injected respectively. Results： The mature DCs were induced by BMMNC co - culturing with relative cytokines, and cell - surface phenotypes were highly expressed. Vaccinating TAA pulsed DC rats had a lower incidence of oncogenesis. There was a significant difference （P 〈 0. 01 ） compare with control group （P 〈0. 01 ） . Conclusion： The mature DC could be obtained from BMMNC. DC could load and submit tumor antigen, which would induce cytotoxic effect for tumor cells invivo. DC vaccine activated T lymphocytes proliferation which induce active immunity against tumor, and it would bring bright prospects in clinical tumor immunotherapy.

关 键 词：树突状细胞 大鼠 卵巢癌 免疫治疗 

分 类 号：R737.31[医药卫生—肿瘤]