小鼠T2细胞特异性转录因子GATA3基因的克隆和序列分析  

Cloning and sequence analysis of specific transcription factor GATA3 of murine T helper 2 type cells

作　　者：方杰[1] 郑金旭[1] 张雅琴[1] 林小涛[1] 吴燕[1] 许化溪[1] 眭建[1] 

机构地区：[1]江苏大学附属医院妇产科,江苏镇江212001

出　　处：《中国妇幼保健》2008年第13期1855-1857,共3页Maternal and Child Health Care of China

基　　金：镇江市社会发展基金(SH2005061)

摘　　要：目的:克隆小鼠Th2细胞分化转录因子GATA3基因的全长编码区cDNA。方法:利用RTPCR方法,从Balb/c小鼠脾细胞的mRNA中扩增GATA3基因全长cDNA,并将其连接到pMD18-T载体上,进行测序和核酸分析。结果:扩增得到的小鼠GATA3基因cDNA全长1332bp,编码444个氨基酸,其结构含有识别和结合DNA所需的高度稳定的蛋白区;blast结果分析表明,该cDNA与Genbank中发表的序列(BC062915)具有100%的同源性。结论:获得小鼠GATA3基因的克隆,为进一步研究转基因免疫干预奠定了基础。Objective： To clone all - length coding eDNA of murine. Methods： Total RNA as well as mRNA were isolated from splenic cells of Balb/c mice and the entire coding eDNA sequence of GATA3 was amplified by reverse transcription polymerase chain reaction （ RT - PCR） . The GATA3 eDNA were cloned in to the pMD18 - T vector. The identification was made by means of restriction enzym eanalysis, PCR and DNA sequencing. Results： The murlne entire coding eDNA of 1 332 bp was amplified from spleen cells. There was 100% homogenous nucleotide sequence as compared with standards equence （BC062915） of GATA3 from Genbank. Conclusion： The eDNA of murine GATA3 is successfully cloned. It will be the basis for studying immune interference of transgenes.

关 键 词：GATA3 基因克隆 RTPCR 

分 类 号：R734.2[医药卫生—肿瘤] R282.710.5[医药卫生—临床医学]