冬虫夏草对抗环磷酰胺致小鼠睾丸氧化损伤的作用  

The effect of Chinese caterpillar fungus on testis oxidative damage induced by cyclophosphamide in the mice

作　　者：靳镭[1] 陈守真[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院生殖医学中心,湖北武汉430030

出　　处：《中国妇幼保健》2008年第13期1858-1860,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨冬虫夏草对抗环磷酰胺致小鼠睾丸氧化损伤的作用。方法:随机将成年昆明小鼠分成3组:正常对照组、模型对照组、冬虫夏草治疗组。模型对照组和冬虫夏草治疗组小鼠按照40mg/kg.d腹腔注射环磷酰胺(cp),连续5天,同时冬虫夏草治疗组小鼠每天给予冬虫夏草混悬液300mg/kg.d灌胃;正常对照组小鼠及模型对照组灌胃等量生理盐水。各组均连续处理28天。末次给药24h后,处死全部小鼠,观察小鼠附睾精子密度、活力、活动率,睾丸组织匀浆后测定超氧化物歧化酶(superoxide dismutas,SOD)、谷胱甘肽过氧化物酶(glutathi0ne Pe roxidase,GSH-Px)、丙二醛(maloniealdehvde,MDA)含量。结果:与模型对照组相比,治疗组精子密度、活力、活动率、睾丸组织SOD与GSH-Px含量等均明显升高(P<0.05、P<0.01),精子畸形率和MDA含量有明显降低(P<0.05、P<0.01)。结论:冬虫夏草可对抗环磷酰胺所致小鼠睾丸生精功能的损害,其作用机制可能与冬虫夏草清除氧自由基,抗氧化作用有关。Objective： To observe the protective effects of cordyceps on the damage of mouse spermatogenesis induced by cydophosphamide. Methods： The mice were randomly divided into normal control, model and eordyeeps treatment groups. Cordyeeps treatment group and control group in accordance with 40 mg· kg^-1 · d^-1 by intrapefitoneal injection of eyelophosphamide in five days, while the eordyeeps treatment group was given with eordyeeps suspension at 300 mg· kg^-1 · d^-1 and the normal group were given with normal saline. The treatment period for all groups was 28 days. All mice were killed to death 24 h after the last medicine administration. Testis homogenate were prepared to measure the level of SOD, GSH - Px, and MDA respectively. Sperm density, sperm motility, and sperm abnormality rate were observed. Results： In eordyeeps treatment group, the level of SOD, GSH -Px and sperm density, sperm motility were increased significantly （P〈0.05, P 〈0.01）, the level of MDA and sperm abnormality rate were decreased significantly （P 〈0〈05, P 〈0.01） . Conclusion： Cordyeeps has a significantly protective effect on spermatogenic damage by eyelophosphamide, the action of eordyceps maybe have relationship with its scavenging free radical and anti - oxidation effects.

关 键 词：冬虫夏草 环磷酰胺 小鼠 生精障碍 抗氧化能力 

分 类 号：R135.13[医药卫生—劳动卫生] R282.71[医药卫生—公共卫生与预防医学]