一种改进的平板影印工具及其应用  

An improved replica plating method and its application to the high-throughput selection of transformants

作　　者：徐民俊[1] 田小群[2] 周世宁[1] 

机构地区：[1]中山大学生命科学学院,有害生物控制与资源利用国家重点实验室,广州510275 [2]广州珠江啤酒股份有限公司,广州510308

出　　处：《微生物学报》2008年第5期657-660,共4页Acta Microbiologica Sinica

基　　金：国家“863计划”(2007AA091904);中国博士后基金(20070410854);广东省自然科学基金(7301731)~~

摘　　要：【目的】建立一种简便高效的平板影印工具,克服传统影印工具的缺陷。【方法】将大量竹签按照一定方法捆扎成与平皿内盖直径相近的捆,将单根牙签转移菌落的效果进行扩大,从而实现菌落的大规模转移。【结果】本实验室使用上述工具成功地进行了平板影印和传代,并进行转化子大规模筛选。【结论】与传统工具相比,该影印工具更为经济简便,而且效果清晰,为微生物平板筛选提供新的方法和思路。国家发明专利申请号:2007100291331。[Objective] To make a simpler and highly efficient replica plating tool to avoid the limitations of the traditional method. [Methods] A number of bamboo-toothpicks was sheaved to form a bundle with diameter close to the plate diameter. The sheaved bamboo-toothpicks were used for replica-plating. [Results] This replica plating tool was successfully used for the screening of Saccahromyces cerevisiae transformants. [Conclusion] Compared with the traditional tool, this tool was simpler and highly efficient.

关 键 词：微生物 平板影印 高效筛选 

分 类 号：Q78[生物学—分子生物学]