晶状体与玻璃体切除术对眼前房相关性免疫偏离影响的实验研究  

The influence of lens and vitreous resection on anterior chamber associated immune deviation in mice model

作　　者：徐建江[1] 孙兴怀[1] 乐琦骅[1] 王艳[1] 王文韬[1] 郑天玉[1] 洪佳旭[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院眼科,上海200031

出　　处：《中华眼科杂志》2008年第4期343-348,共6页Chinese Journal of Ophthalmology

基　　金：上海市科学技术委员会科研计划资助项目（05ZRl4025）

摘　　要：目的探讨大鼠晶状体与玻璃体切除术后前房相关性免疫偏离（ACAID）诱导能力的变化及其与术后时间的关系。方法为实验研究。Wistar大鼠51只，右眼为实验眼，建立晶状体与玻璃体切除模型后单纯随机数字表法随机分为5组，每组10只；余1只Wistar大鼠用于提供诱导ACAID的所需去上皮角膜片。另有SD大鼠5只，用于提供诱导ACAID所需去上皮角膜片。A组为阴性对照组，在术后1周前房植入Wistar大鼠角膜片；B组为阳性对照组，术后1周颈部皮下注射SD大鼠脾细胞，前房不植入角膜片；C、D及E组为实验组，分别在术后1、4及8周前房植入SD大鼠角膜片。B组在颈部皮下注射SD大鼠脾细胞悬液1周后，右耳廓皮下注射SD大鼠脾细胞悬液，以诱导迟发性超敏反应（DTH）。C、D及E各实验组均在前房植入角膜片后2周，右耳廓皮下注射SD大鼠脾细胞悬液，诱导DTH。耳廓膨胀数反映DTH的发生与程度。同时，各组取房水，检测转化生长因子B2（TGF-B2）、白介素10（IL-10）浓度；取心脏血，检测血清IL4与IL-10的浓度；取脾脏检测GATA-3mRNA的水平。实验结束时各组进行组织病理学检查。结果（1）DTH的检测：术后1、4及8周实验组均诱导出DTH。（2）血清IL4测定值A组为（4．073±0．198）ng／L，B组为（5．806±0．635）ng／L，C组（5．535±0．278）．g／L，D组（4．102±0．344）ng／L及E组（5．313±0．317）ng／L；血清IL-10测定值A组为（7．854±2．349）ng／L，B组为（25．633±6．307）ng／L，C组（40．103±16．010）ng／L，D组（14．321±2．983）ng／L及E组（28．620±5．251）ng／L；房水IL-10浓度测定值A组为（8．857±0．401）ng／L，B组为（22．882±3．315）ng／L，C组（21．548±0．477）ng／L，D组（7．742±0．952）ng／L及E组（12．119±0．477）ng／L；房水中TGF-B浓度的测定值A组为（5．800±2．899）ng,／L，B组为（60．010±0．Objective To explore the impact of lens and vitreous resection on the induction of the anterior chamber associated immune deviation （ ACAID ） in the mice, and its relationship with the time duration postoperatively. Methods The current study was an experimental research. Fifty-one Wistar mice were chosen to involve this study. After the surgeries, 50 Wistar mice were randomly divided into 5 groups.Other 5 SD mice and 1 Wistar mouse were used as the providers of epithelium removed corneal grafts to induce ACAID. Group A： corneal grafts from wistar mouse were implanted into the anterior chamber 1 week after the lens and vitreous resection. Group B： the spleen cells were injected into the nape of neck 1 week after the lens and vitreous resection. The corneal grafts from SD mice were implanted into the anterior chamber 1 week, 4 week and 8 week after the lens and vitreous resection in group C, D and E respectively. To induce the delayed type hypersensitivity （ DTH ）, spleen cells were injected into the right ear pinna 1 week after the neck injection in group B, 2 weeks after the corneal grafts anterior chamber implantation in other groups. At the same time point as the DTH inducement, aqueous humor was collected to measure the concentration of transforming growth factor （TGF） -β2 and interleukin （IL）-10; heart blood was collected to detect the concentration of IL-4 and IL-10. And the spleens were removed to evaluate the expression of the GATA-3 mRNA by RT-PCIL The eyeballs were enucleated to evaluate the histopathologic changes. Results （ 1 ） The evaluation of DTH. The DTH were found in group B, C, D and E. （2） The analysis of the TGF- β2 and IL-10 concentrations in aqueous humor and the IL-4, IL-10 concentrations in serum. The concentrations of IL-4 in serum were （4. 073 ±0. 198）ng/L in group A, （5. 806 ±0. 635）ng/L in group B, （5. 535 s0. 278）ng/L in group C, （4. 102 s0. 344） ng/L in group D and （5. 3β s0. 317）ng/L in group E. The concentrations of IL-10 in serum

关 键 词：眼外科手术 玻璃体切除术 血房水屏障 血视网膜屏障 前房 超敏 反应 迟发型 

分 类 号：R686[医药卫生—骨科学]