冷冻消融对肝癌患者外周血CD4^+CD25^+调节T细胞影响的初步研究  

Effect of Cryosurgery on Regulatory CD4^+CD25^+ T Lymphocytes in the Peripheral Blood of Patients with Hepatocellular Carcinoma

作　　者：李小丽[1] 司同国[1] 于海鹏[1] 郭志[1] 

机构地区：[1]天津市肿瘤防治重点实验室天津医科大学附属肿瘤医院介入治疗科,天津市300060

出　　处：《中国肿瘤临床》2008年第8期453-454,共2页Chinese Journal of Clinical Oncology

基　　金：吴阶平医学基金会临床科研专项基金资助(编号:04101001)

摘　　要：目的:探讨氩氦冷冻对肝癌外周血CD4+CD25+调节T细胞的影响及临床意义。方法:原发性肝癌患者20例,其中Ⅱ期14例、Ⅲ期6例,分别采集氩氦冷冻治疗术前及术后1个月外周血,采用流式细胞术检测外周血T淋巴细胞亚群(CD3+T、CD4+T、CD8+T、CD4+T/CD8+T、NK细胞)及Treg细胞。结果:肝癌Ⅲ期患者外周血Treg细胞占CD4+T细胞比例明显高于Ⅱ期患者(6.5%±1.2%、9.1%±2.0%,P=0.013)。与氩氦冷冻治疗前比较治疗后1个月Treg细胞一定程度降低(8.2%±1.1%、7.7%±1.0%),但差异无显著性(P=0.052);CD4+T、CD4+T/CD8+T、NK细胞明显升高,CD8+T细胞降低(P<0.05)。结论:肿瘤负荷可显著促进肝癌患者外周血Treg细胞分化。肝癌氩氦冷冻治疗后短期内Treg细胞比例轻度下降,T淋巴细胞亚群分布异常得到一定改善。Objective： To analyze the effect of Argon-Helium Cryosurgery （AHCS） on the differentiation of regulatory CD4＋ CD25＋ T cell（Treg） and its implication in patients with hepatocellular careinoma（HCC）. Methods： Peripheral venous blood samples were obtained from 20 patients with HCC befure and 1 month after AHCS. Treg cells and T lymphocyte subsets in the peripheral blood were measured by flow cytometry. Results： The percentage of Treg cells in stage Ⅲ patients were higher than in stage Ⅱ patients （P〈0.05）. After AHCS, the percentage of Treg cells was deereased, but with nn significant difference（P〉0.05）. CD4＋T, NK and CD4＋T/CD8＋T were significantly increased after AHCS（P〈0.05）. Conclusion： Tumor load can significantly promote the differentiation of Treg cells in patients with HCC. After the therapy of AHCS, the per- centage of Treg has no significant changes in a short time. The abnormal distribution of subsets of T-lymphocytes can be improved.

关 键 词：肝癌 氩氦冷冻 CD4^+CD25^+调节T细胞 T淋巴细胞 

分 类 号：R735.7[医药卫生—肿瘤]