Bax蛋白和Fas抗原在骨巨细胞瘤中的表达及意义  

Expression of Bax protein and Fas antigen in giant cell tumor of bone

作　　者：肖万军[1] 赵政军[1] 

机构地区：[1]沈阳市第四人民医院骨外科,沈阳110001

出　　处：《临床肿瘤学杂志》2008年第4期332-334,共3页Chinese Clinical Oncology

摘　　要：目的:通过检测骨巨细胞瘤(giant cell tumor of bone,GCT)中Bax蛋白和Fas抗原的表达,探讨Bax蛋白和Fas抗原与GCT临床病理之间的关系,以指导临床治疗。方法:应用免疫组织化学技术EnVision微波二步法检测了42例GCT中Bax蛋白和Fas抗原的表达情况,按照WHO(2003)分级标准,Ⅰ级18例,Ⅱ级15例,Ⅲ级9例,细胞浆或膜呈棕黄色至深棕黄色颗粒为阳性细胞,切片中阳性细胞数≥10%为阳性病例,阳性细胞数<10%为阴性病例。结果:GCT中Bax蛋白的阳性率为50%,其中Ⅰ、Ⅱ、Ⅲ级阳性率分别为66.70%、46.47%和22.22%,Ⅰ级与Ⅱ、Ⅲ级比较,差异具有显著性(P<0.05)。GCT中Fas抗原的阳性率为54.76%,其中Ⅰ、Ⅱ、Ⅲ级阳性率分别为77.78%、40.00%和33.34%,Ⅰ级与Ⅱ、Ⅲ级比较,差异具有显著性(P<0.05)。结论:Bax蛋白和Fas抗原的表达与GCT病理分级密切相关,低表达可能是骨巨细胞瘤术后易复发和对化疗不敏感的重要原因之一。Objective：To investigate the expression and significance of Bax protein and Fas antigen in giant cell tumor of bone. Methods ：We detected the expression of Bax protein and Fas antigen of 42 cases by immunohistochemical method. There are 18 cases of rank Ⅰ ,15 cases of rank Ⅱ and 9 cases of rank Ⅲ according to WHO（2003） criteria. Postive signals were shown in cytoplasma and cell membrane. Results：The positive rate of Bax protein is 50%, which of class Ⅰ , Ⅱ and Ⅲ were 66. 70%, 46. 47% and 22. 22%, respectively. There was significantly different between class Ⅰ and the two other classes（P 〈0. 05）. The positive rate of Fas antigen is 54. 76%, which of class Ⅰ , Ⅱ and Ⅲ were 77. 78%, 40. 00% and 33.34%, respectively. There was significantly different between class Ⅰ and the two other classes （ P 〈 0. 05 ）. Conclusion ： Abnomal expression of Bax protein and Fas antigen in bone giant cells tumor of is one of the most important reasons to recur after operations and insensitive to chemotherapy.

关 键 词：骨巨细胞瘤 BAX蛋白 FAS抗原 

分 类 号：R738.1[医药卫生—肿瘤]