三七总皂甙抗肺缺血再灌注损伤作用机制的实验研究  

An Experimental Study of Protective Effects of Panax Notoginseng Saponins During Lung Ischemia/reperfusion Injury in Rabbits

作　　者：张晓隆[1] 王方岩[1] 徐正祄[1] 王万铁[1] 郝卯林[1] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035

出　　处：《中国微循环》2008年第2期73-75,共3页Journal of Chinese Microcirculation

基　　金：浙江省卫生厅科研基金资助项目(NoSWS00021)

摘　　要：目的探讨三七总皂甙(panax notoginseng saponins,PNS)抗肺缺血再灌注损伤作用及其机制。方法复制在体兔肺缺血再灌注损伤模型。30只日本大耳兔,随机均分为三组:假手术组(S组),缺血再灌注组(IR组)和缺血再灌注+三七总皂甙组(PNS组)。取肺组织测湿/干重比(W/D),计算肺泡损伤率(IAR)。电镜观察细胞超微结构改变。原位杂交法检测肺组织蛋白激酶C(PKCα、δ、θmRNA)表达。结果IR组和PNS组的W/D与IAR均高于S组(分别P<0.05和P<0.01),但PNS组显著低于IR组(P<0.01)。IR组肺组织的超微结构损伤严重,PNS组损伤程度明显较轻。原位杂交发现PNS组肺小静脉PKCα、δ、θmRNA表达均显著高于IR和S组(分别P<0.05,P<0.01)。结论三七总皂甙可上调LIRI时肺组织PKCα、δ、θmRNA的表达,减轻LIRI发挥积极作用。Objective To observe protective effects of panax notoginseng saponins（PNS） during lung ischemia reperfusion injury（LIRI） and to investigate its mechanism. Methods Rabbit lung model of ischemia reperfusion injury was constituted in vivo. 30 rabbits were randomly divided into three groups： sham operation group（ S group）, ischemia-reperfusion group（ IR group）and ischemia-reperfusion plus panax notoginseng saponins group（ PNS group）. Wet to dry weight ratio（W/D）, injured alveoli rate（IAR） in lung tissue were examined, ultramicroscopic structure changes were observed at the end of experiment. Meanwhile the location and expression of PKC mRNA were observed. Results The value of W/D and IAR were much higher in IR group, but decreased in PNS group. Electron microscope showed obvious uhrastructure injury brought by LIRI in IR group, which was greatly attenuated in PNS group. The average optical density values of PKCα、δandθmRNA in small pulmonary veins in PNS group were significantly higher than those in IR group and S group. Conclusion Panax notoginseng saponins produces notable protective effects on LIRI in rabbits by activating PKCα、δandθ mRNA expression in lung tissue.

关 键 词：肺 三七总皂甙 缺血再灌注损伤 蛋白激酶C 

分 类 号：R363[医药卫生—病理学]