IL-6对甲状腺相关眼病分化后眼眶脂肪细胞瘦素mRNA表达的影响  

IL-6 on expression of leptin mRNA in orbital differentiated adipocytes of thyroid associated ophthalmopathy

作　　者：唐建[1] 罗清礼[2] 唐莉[2] 

机构地区：[1]四川省成都市爱尔眼科医院,610031 [2]四川大学华西医院眼科,四川省成都市610041

出　　处：《眼科新进展》2008年第5期327-329,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30371518)~~

摘　　要：目的探讨白介素-6(interleukin-6,IL-6)对体外培养的甲状腺相关眼病(thyroid associated ophthalm opathy,TAO)分化后眼眶脂肪细胞瘦素mRNA表达的影响。方法取TAO患者行眼眶减压术的眼眶脂肪组织,体外培养眼眶前脂肪细胞,诱导分化,采用0.5μg·L-1、5μg·L-1和50μg·L-1的IL-6处理分化后的脂肪细胞24h(设空白对照组),以及5μg·L-1的IL-6分别处理分化后的脂肪细胞0h、12h、24h、48h,实时荧光定量PCR检测瘦素mRNA的表达。结果体外培养的眼眶前脂肪细胞在诱导分化刺激下,分化为脂肪细胞;0.5μg·L-1、5μg·L-1和50μg·L-1的IL-6作用24h,瘦素mRNA表达分别为1.61±0.53、0.88±0.62、0.21±0.56,随质量浓度的增大而下降,呈量效依赖性,但与对照组(1.84±0.82)相比,差异没有统计学意义(P>0.05);5μg·L-1的IL-6作用12h、24h、48h,瘦素mRNA表达分别为1.43±0.46、1.05±0.62、0.20±0.75,随时间的延长而下降,呈时效依赖性,但与0h组(1.73±0.31)相比,差异没有统计学意义(P>0·05)。结论IL-6对TAO分化后眼眶脂肪细胞的瘦素mRNA表达有下调作用,但无统计学意义。Objective To investigate the effect of interleukin-6 （IL-6） on the expression of lepttn mRNA in orbital differentiated adipocytes of thyroid associated ophthalmopathy （TAO）. Methods Preadipocytes from orbital tissues of TAO patients after orbital decompression were cultured and committed to differentiate. The differentiated orbital adipocytes were incubated with 0.5 μg · L^-1 ,5 μg · L^-1 and50 μg · L^-1 of IL-6 for 24 hours or 5 μg · L^-1IL-6 for 0 hour,12 hours,24 hours, and 48 hours, respectivley. The expression of leptln mRNA in the differentiated adipocytes was detected by real-time fluorescent quantitative PCR Results Induced preadipocytes differentiated into the cells with morphological feature of mature adipocyte under the appropriate in vitro condition with the red staining for oil red.After treated with 0.5 μg · L^-1 ,5 μg · L^-1 and 50 μg · L^-1 of IL-6 for 24 hours,the expressions oflepttn mRNA in the differentiated adipocytes were 1.61 ±0.53,0.88 ±0.62 and 0.21 ±0.56,respectively.The expression of leptin mRNA was decreased with the mass concentration of IL-6 increases, and quantity-effect dependence was fotmd. There was no statistical difference with the control group（ 1.85 ± 0.82 ,P 〉 0.05）. The expression of lepttn mRNA in the differentiated adipocytes was 1.43 ± 0.46,1.05 ± 0.62, and 0.20 ± 0. 75 after treated with 5 μg · L^-1 of IL-6 treatment for 12 hours,24 hours and 48 hours, respectively. The expression of leptin mRNA decreased with the time lasting increased ,and time-effect dependence was found. However, the changes of expression ofleptin mRNA were not significantly different from 0 hour of IL-6 treatment group （ 1.73 ±0.31, P 〉 0.05 ）. Conclusion IL-6 can suppress the expression of leptin mRNA in the differentiated adipocytes of TAO subjects, but statistical significance has not yet proved.

关 键 词：甲状腺相关眼病 白介素-6 瘦素 脂肪细胞 实时荧光定量PCR 

分 类 号：R777.5[医药卫生—眼科]