高温海藻糖合酶产生菌的诱变选育及产酶条件研究  

BREEDING OF THERMOSTABLE TREHALOSE SYNTHASE-PRODUCING STRAIN AND STUDYING ON ITS ENZYME PRODUCTION

作　　者：陈红漫[1] 訾晓男[1] 阚国仕[1] 马晶[1] 

机构地区：[1]沈阳农业大学生物科学技术学院辽宁省酶工程技术中心,辽宁沈阳110161

出　　处：《食品研究与开发》2008年第5期5-8,共4页Food Research and Development

基　　金：辽宁省自然科学基金项目资助(2040515)

摘　　要：通过紫外线复式诱变,筛选到一株高产高温海藻糖合酶的突变菌株SN02004-01-3,与出发菌株Bacillus sp.SN02004-01相比,其产酶能力提高了3.6倍。传代试验结果证明:突变菌株SN02004-01-3具有良好的遗传稳定性。突变株发酵条件优化后表明:最适培养时间为16h,最适pH为7.0,最适生长温度为70℃。The mutant strain SN02004-01-3 with relatively high thermostable trehalose synthase was isolated. The mutant＇s ability to produce thermostable trehalose synthase increased 3.6 times after treating with three times UV. Transfer of culture experiment showed that it can maintain stability to some extent, and the fermenta- tion properties were different. The maximum medium thermostable trehalose synthase activity reached at 70 ℃ and pH 7.0 after 16 h culture.

关 键 词：BACILLUS sp.SN02004-01菌株 紫外线诱变 产酶条件 高温海藻糖合酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]