大鼠正畸牙移动中牙周组织内STRO-1动态变化的研究  

Changes of STRO-1 in Periodontal Tissue of Orthodontic Tooth Movement in Rats

作　　者：王光 丁寅 潘峰 张玉 许一起 梁莉 

机构地区：[1]第四军医大学口腔医院正畸科,陕西西安710032 [2]海军91551部队门诊部

出　　处：《口腔医学研究》2008年第2期124-126,共3页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:30572069)

摘　　要：目的:通过观察大鼠正畸牙移动中牙周组织内STRO-1的动态变化,研究大鼠牙周膜干细胞(periodontal ligament stem cell,PDLSC)在正畸牙周组织改建中的作用及表达变化的规律。方法:选用幼年SD大鼠,建立正畸牙移动的模型,分别在加力后1、3、5、7、10、14 d处死动物,制备标本。应用EnVision系统两步法和图像分析进行半定量分析。结果:正常大鼠牙周组织中STRO-1表达较低。STRO-1在加力组牙周膜中的表达与对照组比较,差异有显著性,在正畸牙移动过程中STRO-1阳性显色细胞明显增多。结论:STRO-1的表达强度与牙周改建的活跃程度相关,揭示了在正畸力作用下牙周组织改建的可能机制和牙周膜干细胞的作用。Objective： To observe the distribution and changes of periodontal ligament stem cell （PDLSC）in rat periodontal tissues, and analyze the role of PDLSC in the periodontal tissue remodeling using cell - surface markers for mesenchymal stem cells during orthodontic tooth movement. Methods： The model of orthodontic tooth movement was established in young SD rats. The animals were sacrificed after 1,3,5,7,10,14 days. The distribution and changes of STRO - 1 in the periodontal tissues of the first molar were observed by immunohistochemistry technology in the section. The optical density （OD） of STRO - 1 was analyzed by image -pro plus6.0 software. Results： Significant difference of the expression STRO - 1 in the periodontal tissues between normal group and experimental group was found. Conclusion： These results indicate that PDLSC participates in the periodontal tissue remodeling during orthodontic tooth movement.

关 键 词：牙周膜干细胞 STRO-1 牙齿移动 牙周组织 免疫组化 

分 类 号：R783[医药卫生—口腔医学]