自然流产孕妇外周血染色体着丝粒点变异与单纯疱疹病毒感染的关系研究  

The study of relationship between aberration of chromosomal centromeric dots and infection of herpes simplex virus in the peripheral blood of pregnant woman with spontaneous abortion

作　　者：刘彦慧[1] 何永明[1] 宋娟[1] 王明波[1] 黎丽芬[1] 石静[1] 

机构地区：[1]中山大学附属东华医院医学遗传研究室,广东523110

出　　处：《国际检验医学杂志》2008年第4期300-302,共3页International Journal of Laboratory Medicine

基　　金：广东省东莞市科技局医学科研项目(20060315)

摘　　要：目的研究单纯疱疹病毒(HSV)感染与孕妇外周血淋巴细胞染色体着丝粒点变异的关系,分析染色体着丝粒点变异导致胚胎发育异常的机制。方法选择早期自然流产孕妇59例,抽取静脉血,采用ELLASA法检测单纯疱疹病毒抗体,阳性患者(IgG-/IgM+或IgG+/IgM+)以常规方法制作外周血淋巴细胞染色体,并采用同步银染法观察染色体着丝粒点变异情况,采用SAS. P9.0统计软件分析单纯疱疹病毒感染与染色体着丝粒点变异率的相关性。另选择10例为健康对照组。结果共有21例早期流产孕妇外周血单纯疱疹病毒抗体阳性,其外周血染色体着丝粒点变异几率显著高于健康对照组,差异有统计学意义(P<0.0001)。结论单纯疱疹病毒可引起外周血染色体着丝粒点变异,导致体细胞有丝分裂时染色体复制错误或丢失而形成非整倍性畸变,由此可以推断单纯疱疹病毒同样可影响生殖细胞减数分裂或胚胎细胞正常分裂,是引起孕妇于孕早期胚胎异常的原因之一。Objective To study the relationship of the aberration of chromosomal centromeric dots （Cd） of peripheral blood and infection of herpes simplex virus （HSV） for pregnant women with spontaneous abortion, and to analyze the mechanism of the aberration of chromosomal centromeric dots inducing embryonic maldevelopment. Methods 59 cases of pregnant women with a history of spontaneous abortion （observation group） and 10 healthy women （set as control group） were enrolled in the study. The peripheral blood HSV antibody was examined with ELLASA technique. The subjects with HSV antibody positive （IgG-/IgM＋ or IgG＋/IgM＋） were examined further for chromosomal centromeric dots by simultaneous silver staining technique. The correlation of HSV infection and the aberration rate of chromosomal centromeric dots was analyzed by applying SAS. P9. 0 software. Results 21out of 59 cases were infected with HSV. The aberration rate of chromosomal centromeric dots was much higher in observation group than that of control group, and there was significant difference between the two groups （P〈0. 000 1）. Conclusion HSV may induce aberration of chromosomal centromeric dots during mitosis, which further leads to chromosome replication error or loss resulting in aneuploid aberration. It concludes that HSV influences on germocyte maiosis or normal embryonic cell division as one of causes for embryonic abnormality at early trimester of pregnancy.

关 键 词：流产 自然 单纯疱疹病毒属 染色体畸变 血液 

分 类 号：R714.2[医药卫生—妇产科学]